In this downloadable poster, we report on the development of a novel assay for functional assessment of the positive complement regulator Properdin. We demonstrate a variability in properdin function in healthy controls, which emphasizes the need for functional complement assays in determining patients’ normal range, elucidating disease cause, and evaluating drug efficacy.
The presented findings have shown that the novel Factor P Functional assay is a versatile tool that can be used to specifically study the function and inhibition of the AP amplification loop and its critical components in an exploratory setting. The successful inhibition of the AP C3-convertase, assembled on the immobilized properdin in vitro, with varied and specific inhibitors, makes it a valuable tool in drug development targeting the amplification loop
