This scientific talk, presented at PEGS 2021, presents a highly sensitive iLite® reporter assay, providing a highly sensitive, rapid, precise method for quantifying NAb response to a wide range of recombinant AAV vectors.
A highly sensitive iLite® reporter assay based on a packaging cell line expressing a recombinant virus containing a tag sequence within the ITRs and a serotype-specific cap gene recognized specifically by a reporter cell line stably transfected with a luciferase reporter-gene placed under the control of an AAV-responsive chimeric promoter will be described.