Wieslab® ASCA test is an ELISA for semi-quantitative/or qualitative detection of IgG autoantibodies against the mannan structure of Saccharomyces cerevisiae in human sera.

The assay is used to detect antibodies in a single serum specimen and the results of the assay are useful as an aid in the diagnosis of Crohn's disease as well as in its differentiation from Ulcerative colitis.

40 patient samples can be tested in duplicate quantitatively alternatively 45 patient samples qualitatively.

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At a glance

Product code ASCA151
Assay target ASCA antibodies
Size Break apart microtitration strips (12x8) 96 wells
Technology ELISA
Read-out of results Qualitative, Semi-quantitative,
Range 10-160 U/mL
Assay time 2h
Detection system ALP/pNPP read at 405 nm
Regulatory status IVD, CE labeled

What's in the box?

  • One frame with break apart wells (12x8)
  • 1.5 mL Negative control (NC), human serum
  • 1.5 mL Positive control (PC), human serum
  • 13 mL Conjugate
  • 2 x 32 mL Diluent (Dil)
  • 13 mL Substrate pNPP-solution
  • 30 mL Wash solution 30x concentrated
  • Five calibrators containing human serum in diluent

All reagents in the kit, except the wash solution, are ready for use and should be stored at 2-8° C.


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