The Wieslab® Capture MPO-ANCA test kit is an ELISA for detection and quantitation of IgG antibodies to myeloperoxidase (MPO) in human sera.

The assay is intended for use in patients with signs and symptoms consistent with microscopic polyangiitis (MP). The results of the assay are to be used as an aid to the diagnosis of microscopic polyangiitis (MP). It is not intended for screening a healthy population

Measurement of MPO-specific ANCA is an important adjunct to clinical findings in the evaluation of clinical subtypes within the systemic vasculitides spectrum.

The Wieslab Capture MPO-ANCA is calibrated against the AF-CDC international standard (Reference Human Serum 15, code IS2720).

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At a glance

Product code CAP MPO IU
Assay target MPO-ANCA
Size 96 wells
Technology ELISA
Read-out of results Quantitative,
Range 0-200 IU/mL
Assay time 2h
Detection system ALP/pNPP read at 405 nm
Regulatory status IVD, CE labeled

What's in the box?

  • One frame with 96 wells
  • 1.5 mL negative control (NC) containing human serum in diluent
  • 1.5 mL positive control (PC) containing human serum in diluent
  • 13 mL Conjugate
  • 2 x 32 mL Diluent (Dil)
  • 13 mL Substrate pNPP
  • 13 mL Stop Solution
  • 30 mL Wash solution 30x concentrated
  • Six calibrators (five calibrators, Cal 2-6, containing human serum) in diluent. 1.5 mL Cal 1 = 0 IU/mL, 1.5 mLCal 2 = 2 IU/mL, 1.5 mL Cal 3 = 10 IU/mL, 1.5 mL Cal 4 = 30 IU/mL, 1.5 mL Cal 5 = 100 IU/mL, 1.5 mL Cal 6 = 200 IU/mL.

All reagents in the kit are ready for use except wash solution and should be stored at 2-8° C.


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