WIESLAB® Vasculitis Screen


The Wieslab® Vasculitis Screening Test Kit is an ELISA for the qualitative detection of antibodies to glomerular basement membrane (GBM), Proteinase-3 (PR3) and Myeloperoxidase (MPO) in human sera.

The assay is used to detect antibodies in a single serum specimen and the results of the assay are to be used as an aid to the diagnosis of reno-pulmonary syndromes and rapidly progressive glomerulonephritis, especially Goodpasture syndrome (GP), Wegener’s granulomatosis (WG/ granulomatosis with polyangiitis) and microscopic polyangiitis (MP).

The assay is intended for use in patients with signs and symptoms consistent with GP, WG, and MP. It is not intended for screening a healthy population and a positive result should always be confirmed by a quantitative assay.

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WIESLAB®  Vasculitis Screen
WIESLAB®  Vasculitis Screen

At a glance

Product code GCP-CAP
Assay target GBM antibodies
Assay target MPO-ANCA
Assay target PR3-ANCA
Size 96 wells
Technology ELISA
Read-out of results Qualitative,
Assay time 40 min
Detection system ALP/pNPP read at 405 nm
Regulatory status IVD, CE labeled

What's in the box?

  • One frame with 96 wells coated with purified GBM antigen (Bovine), monoclonal anti-proteinase 3/proteinase 3 (Human Neutrophil) and monoclonal anti-myeloperoxidase/myeloperoxidase (Human Neutrophil) and control wells with monoclonal mouse antibody or human serum albumin: one lid sealed in a foil pack with a dry pack.
  • 3 mL negative control (NC) containing human serum in diluent (green colour)
  • 3 mL positive control (PC) containing human serum in diluent (red colour)
  • 13 mL conjugate containing alkaline phosphatase-labelled (Goat) antibodies to human IgG (blue colour)
  • 32 mL Diluent (Dil) containing PBS (red colour)
  • 13 mL Substrate pNPP
  • 13 mL Stop Solution
  • 30 mL Wash solution 30x concentrated

All reagents in the kit are ready for use except wash solution and should be stored at + 2-8° C.


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