WIESLAB® PR3-ANCA
PR3 IU
The Wieslab® PR3-ANCA test kit is an ELISA for detection and quantitation of IgG antibodies to proteinase 3 (PR3) in human sera.
The assay is used to detect antibodies in a single serum specimen and the results of the assay are to be used as an aid to the diagnosis of granulomatosis with polyangiitis (Wegener’s granulomatosis).
Approximately 80-90% of patients with Wegener’s granulomatosis manifest PR3-ANCA. PR3-ANCA also occurs in other systemic vasculitides, e.g. microscopic polyangiitis and focal and extracapillary necrotizing glomerulonephritis.
At a glance
| Product code | PR3 IU |
| Assay target | PR3-ANCA |
| Size | 96 wells |
| Technology | ELISA |
| Read-out of results | Quantitative, |
| Range | 0-200 IU/mL |
| Assay time | 2h |
| Detection system | ALP/pNPP read at 405 nm |
| Regulatory status | IVD, CE labeled |
What's in the box?
- One frame with 96 wells
- 1.5 mL Negative control (NC) containing human serum in diluent
- 1.5 mL Positive control (PC) containing human serum in diluent
- 13 mL Conjugate
- 2 x 32 mL Diluent (Dil)
- 13 mL Substrate pNPP
- 13 mL Stop solution
- 30 mL Wash solution 30x concentrated
- Six calibrators (five calibrators, Cal 2-6, containing human serum) in diluent. 1.5 mL Cal 1 = 0 IU/mL, 1.5 mL Cal 2 = 2 IU/mL, 1.5 mL Cal 3 = 10 IU/mL, 1.5 mL Cal 4 = 30 IU/mL, 1.5 mL Cal 5 = 100 IU/mL, 1.5 mL Cal 6 = 200 IU/mL
All reagents in the kit are ready for use except wash solution and should be stored at 2-8° C.
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