The Wieslab® PR3-ANCA test kit is an ELISA for detection and quantitation of IgG antibodies to proteinase 3 (PR3) in human sera.

The assay is used to detect antibodies in a single serum specimen and the results of the assay are to be used as an aid to the diagnosis of granulomatosis with polyangiitis (Wegener’s granulomatosis).

Approximately 80-90% of patients with Wegener’s granulomatosis manifest PR3-ANCA. PR3-ANCA also occurs in other systemic vasculitides, e.g. microscopic polyangiitis and focal and extracapillary necrotizing glomerulonephritis.

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At a glance

Product code PR3 IU
Assay target PR3-ANCA
Size 96 wells
Technology ELISA
Read-out of results Quantitative,
Range 0-200 IU/mL
Assay time 2h
Detection system ALP/pNPP read at 405 nm
Regulatory status IVD, CE labeled

What's in the box?

  • One frame with 96 wells
  • 1.5 mL Negative control (NC) containing human serum in diluent
  • 1.5 mL Positive control (PC) containing human serum in diluent
  • 13 mL Conjugate
  • 2 x 32 mL Diluent (Dil)
  • 13 mL Substrate pNPP
  • 13 mL Stop solution
  • 30 mL Wash solution 30x concentrated
  • Six calibrators (five calibrators, Cal 2-6, containing human serum) in diluent. 1.5 mL Cal 1 = 0 IU/mL, 1.5 mL Cal 2 = 2 IU/mL, 1.5 mL Cal 3 = 10 IU/mL, 1.5 mL Cal 4 = 30 IU/mL, 1.5 mL Cal 5 = 100 IU/mL, 1.5 mL Cal 6 = 200 IU/mL

All reagents in the kit are ready for use except wash solution and should be stored at 2-8° C.


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