Sampling Instructions

4 things to think about when preparing samples for Wieslab Diagnostic Services:

  • The specimen requirements are written on our request forms and specifies the requested volume, storage temperature, and any special handling considerations.
  • For panels or multiple assay requests, the labelled sample should be submitted together with the completed request form. Tests will be performed in the order of that priority.
  • Don´t forget to state the referring physician´s name and phone number on the request form clearly. This is particularly important for acute testing.
  • The specimen should be properly processed, packaged and transported to the laboratory in a timely manner and under environmental conditions that will not compromise the integrity of the specimen.

Using the right sample is key to getting a valid result.

If you have urgent questions regarding instructions for specimen collection, contact us on phone +46 40 53 76 60 or email analysis.se@svarlifescience.com

Specific sampling instructions

Autoantibody assays

At times when the sample is not directly sent, the sample should be stored in a refrigerator (about 2-8 °C for up to one week) or freezer (about -20 °C for prolonged storage) awaiting transport.

Blood sampling

  • Blood should be collected in plain tubes (serum tubes) without coagulants or other additives.
  • Both venous and capillary samples are acceptable.
  • 3 mL serum (7 mL blood) will be enough for approximately 15 tests.
  • Usually one tube is enough.

Serum

  • The sample should be coagulating at least 30 minutes and then centrifuged at ambient temperature
  • Separate serum into plain tube/s
  • Serum should be kept cold until transport
  • Transport at room temperature, standard postal letter is acceptable (padded envelope marked UN 3373)

CSF (Cerebrospinal fluid)

3 mL CSF will be enough for approximately 10 tests. It is not necessary to freeze these samples before transport. The samples can be sent in padded envelopes in room temperature.

Complement assays

The serum samples and EDTA-plasma should be frozen and shipped on dry ice.

Serum (C3, C4, C1q and functional complement assay)

Serum samples for complement analysis (C3, C4, C1q, and functional complement assay) should be collected in plain tubes (serum tubes) without coagulants or other additives.

  • Turn the test tube at least 5 times after sampling
  • The sample should be coagulating for at least 30 minutes and then centrifuged 10 minutes
  • Separate serum into two plain tubes and transport it on dry ice

EDTA-plasma (C3d)

  • The sample should be centrifuged for 10 minutes at ambient temperature
  • Separate plasma into a plain tube and transport it on dry ice – within 24 hours of sampling

Biomarkers (in CSF)

Always use polypropylene tubes for collecting, centrifuging and transportation.

Adults: Collect the first 10-12 mL CSF

Children (<16 years): Collect the first 3 mL CSF

  • In case of heavy bleeding, discard the first mL CSF, and note this on the request form
  • Samples should be centrifuged at 1300-1800g for 10 min
  • The supernatant should be transferred to a new polypropylene tube before aliquoting as described below:

Alzheimer markers and markers of neuronal damage (Parenchymal): At least 0.5 mL CSF divided in 3 polypropylene cryotubes and frozen.

Neuromyelitis Optica Spectrum Disease: At least 0.5 mL CSF divided in 2 polypropylene cryotubes and frozen.

The samples must be packed with dry ice when shipped.

NOTE! Samples must not be thawed after freezing and never send frozen samples on Fridays.

Narcolepsy assay

For this assay, the following samples are needed:

  • Serum: 3 mL (Trib2)
  • CSF: 0,5 mL (Orexin/Hypokretin) and 
  • EDTA-plasma: 2,5 mL (HLA-DQB*06:02)* 

The sample should be stored in a refrigerator (about 2-8 °C for up to one week) awaiting transport. Transport at room temperature, standard postal letter is acceptable (padded envelope marked UN 3373).

* NOTE! For the HLA test a signed Informed Consent form needs to be enclosed with the sample. The Informed Consent Form can be downloaded here.

Therapeutic Drug Monitoring

The sample is preferably collected the same day as the next planned drug injection/infusion but before drug administration.

Cmin is the minimal drug concentration at steady state and when the variations of drug levels in serum are the lowest.

The levels of neutralizing anti-drug antibodies are not accurately determined with these methods if sera contain detectable levels of drug.

Take a venous or capillary blood sample in a tube without additive, let the sample clot for approximately 30 minutes and centrifuge it.

Send serum by ordinary mail or on dry ice, please see the instructions after each sample number below for the correct transport condition. Cold packs are recommended for international transport of specimen that can be sent in “room temperature”.

If the sample cannot be sent immediately it should be stored cold (2-8° C) in a refrigerator for up to one week or freezer (-20° C) until shipment.

Get in touch with our Laboratory!

Don´t hesitate to contact us regarding any questions about our sampling instructions or diagnostic testing  services.