Complement Factor P Functional Assay
COMPL FPF RUO
Zoom in on the amplification loop of the alternative pathway with this functional ELISA for Factor P (Properdin) — ideal for understanding regulatory dynamics and evaluating complement-modulating therapies.
- Reliable, objective, and easy-to-interpret results
- Results in just a few hours
- Low inter-assay variability and excellent reproducibility
- Developed by our in-house experts in collaboration with key opinion leaders
- Ready-to-use reagents for a streamlined workflow
- Excellent specificity means precise results without false positives
Product Overview
The Factor P Functional assay is an enzyme-linked immunosorbent assay (ELISA) that combines principles of the functional complement activity assays with the use of labelled antibodies specific for deposited complement proteins. The amount of deposited complement proteins is proportional to the functional activity of Factor P in the sample.
The purpose of the product is to determine the function of Factor P in test samples. The functionality is determined qualitatively in relation to the calibrator (human serum) set to be 100% functional.
FOR RESEARCH USE ONLY. Not for use as a diagnostic tool or in the management of patients.
How it works
Principle of the functional factor P assay
Factor P (FP) from the sample is bound by a capture antibody on the ELISA.
What's in the box?
- One frame with microtiter wells (12x8)
- 1.5 mL Negative control (NC)
- 3.0 mL Calibrator, 200 ng/mL
- 32 mL Diluent (Dil), red color
- 30 mL Wash solution, 30x concentrated
- Activator, lyophilized
- 15 mL Activator Diluent
- 3.0 mL Quench
- 7.5 mL Conjugate containing HRP-labelled antibody directed against C3b
- 15 mL Substrate TMB
- 15 mL Stop solution (0.5M H2SO4)
Please note: All reagents in the kit are ready for use except washing solution and activator. All reagents should be stored at 2-8°C, except for the Activator. When stored at 2-8°C, the diluted wash solution is stable until the date of expiration for the kit.
Specifications
| Product code | COMPL FPF RUO |
| Assay target | Properdin |
| Size | 96 well |
| Technology | ELISA |
| Detection system | HRP/TMB read at 450 nm |
| Regulatory status | Research Use Only |
| Range | 0 - 200% relative activity |
| Assay time | 4h |
