Complement Factor P Quantitative Assay
COMPL FPQ RUO
Zoom in on the amplification loop of the alternative pathway with this quantitative ELISA for Factor P (Properdin) — ideal for understanding regulatory dynamics and evaluating complement-modulating therapies.
- Reliable, objective, and easy-to-interpret results
- Results in just a few hours
- Low inter-assay variability and excellent reproducibility
- Developed by our in-house experts in collaboration with key opinion leaders
- Ready-to-use reagents for a streamlined workflow
- Excellent specificity means precise results without false positives
Product Overview
The Factor P Quantitative Assay is a sandwich ELISA for quantifying human Factor P in, for example, serum or plasma samples. The assay is based on immobilized antibodies in a 96-well plate, which capture Factor P present in test samples incubated in the plates. The bound Factor P is detected with a secondary antibody labelled with HRP. The HRP subsequently catalyzes a cleavage of the substrate that induces a color change correlating with the concentration of Factor P in the test sample.
The purpose of the product is to quantitate the amount of human Factor P in test samples.
The product is intended for use by trained laboratory personnel. The results shall not be used for clinical diagnosis or patient management. FOR RESEARCH USE ONLY.
How it works
Principle of the quantitative factor P assay
The wells of the ELISA are coated with a capture antibody that binds to Factor P (FP) in the sample.
What's in the box?
- One frame with microtiter wells (12x8) coated with anti-Factor P monoclonal antibody
- 3.0 mL vial with Calibrator (200 ng/mL). Ready to use. The Calibrator must be further diluted according to instructions in 9.1.2.
- 1.5 mL Low control (LC)
- 1.5 mL High control (HC)
- 2x32 mL Diluent (Dil)
- 30 mL Wash solution, 30x concentrated
- 15 mL Conjugate containing HRP-labelled antibody directed against Factor P
- 15 mL Substrate TMB
- 15 mL Stop solution (0.5M H2SO4)
Please note: All reagents in the kit are ready for use except washing solution. The reagents should be stored at 2-8°C. When stored at 2-8°C, the diluted Wash solution is stable until the date of expiration for the kit.
Specifications
| Product code | COMPL FPQ RUO |
| Assay target | Properdin |
| Size | 96 well |
| Technology | ELISA |
| Detection system | HRP/TMB read at 450 nm |
| Regulatory status | Research Use Only |
| Range | 0 - 200 ng/mL |
| Assay time | 2h |
