Complement Factor P Quantitative Assay
COMPL FPQ RUO
The Factor P Quantitative Assay is a sandwich ELISA for quantifying human Factor P in, for example, serum or plasma samples. The assay is based on immobilized antibody in a 96-well plate, which captures Factor P present in test samples incubated in the plates. The bound Factor P is detected with a secondary antibody labelled with HRP. The HRP subsequently catalyzes a cleavage of the substrate that induces a color change correlating with the concentration of Factor P in the test sample.
The purpose of the product is to quantitate the amount of human Factor P in test samples.
Want to know more about the principle of the quantiative Factor P assay? Click to view an animation.
The product is intended for use by trained laboratory personnel. The results shall not be used for clinical diagnosis or patient management. FOR RESEARCH USE ONLY.
At a glance
| Product code | COMPL FPQ RUO |
| Assay target | Properdin |
| Size | 96 well |
| Technology | ELISA |
| Read-out of results | Quantitative, |
| Range | 0 - 200 ng/mL |
| Assay time | 2h |
| Detection system | HRP/TMB read at 450 nm |
| Regulatory status | Research Use Only |
What's in the box?
- One frame with microtiter wells (12x8) coated with anti-Factor P monoclonal antibody
- 3.0 mL vial with Calibrator (200 ng/mL). Ready to use. The Calibrator must be further diluted according to instructions in 9.1.2.
- 1.5 mL Low control (LC)
- 1.5 mL High control (HC)
- 2x32 mL Diluent (Dil)
- 30 mL Wash solution, 30x concentrated
- 15 mL Conjugate containing HRP-labelled antibody directed against Factor P
- 15 mL Substrate TMB
- 15 mL Stop solution (0.5M H2SO4)
Please note: All reagents in the kit are ready for use except washing solution. The reagents should be stored at 2-8°C. When stored at 2-8°C, the diluted Wash solution is stable until the date of expiration for the kit.
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