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iLite T-Cell Activation Bioassays

A biologically relevant and powerful way to assess CD3 induced T-cell activation

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A biologically relevant way to assess CD3 induced T-cell activation

T-Cell Activation Bioassays

Our T-cell Activation Bioassays, based on Svar’s proprietary iLite® technology, are designed to measure the effectiveness of antibodies engaging with CD3 receptors and triggering T-cell activation – a common mechanism of action (MoA) of therapeutic antibodies.

iLite T-Cell Activation Reporter Gene Assays

Our iLite T-cell activation assays utilize a dual-cell system with luciferase readouts, mimicking the natural interaction of the TCR/CD3 complex. This results in a powerful way of measuring the efficacy of antibodies to elicit T cell activation through CD3 interactions.

  • Easier workflow than traditional T-cell activation Assays
  • "Assay-ready" format for rapid and convenient use
  • A secondary reporter gene that acts as an internal control, compensating for differences in cell number and matrix effects
  • Reduced assay variability

Our T-Cell Activation Reporter-Gene Assays

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ALL Effector cells Target cells
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BM5025

iLite® CD19 (+) Target

Engineered to express CD19, this cell line is ideal for ADCC and bispecific antibody assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5025 | ADCC | T-Cell Activation | Cell-based reporter gene assay | Luminescence |
BM5026

iLite® CD19 (-) Target

CD19-negative target cell line used as a specificity control in ADCC and T cell engager assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5026 | ADCC | T-Cell Activation | Cell-based reporter gene assay | Luminescence |
BM5005

iLite® CD3 Effector

Optimized for quantifying T cell-mediated cytotoxicity and bispecific antibody function via CD3 engagement

CELL-BASED
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Cell-based reporter gene bioassay | T-Cell Activation Cells | BM5005 | T-Cell Activation | Cell-based reporter gene assay | Luminescence |

Didn't find what you were looking for?

We offer products customized to your needs. Our custom iLite® reporter-gene assays can be adapted to almost any target. We also offer custom ELISA plates and other products. In addition, you can take advantage of our service offerings and let us do the work for you.

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