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iLite ADCC & ADCP Bioassays

Effector and Target cells engineered to work optimally together – a system that is greater than the sum of its parts

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Greater than the sum of its parts

ADCC and ADCP Bioassays

Many antibody-based therapeutics rely, at least in part, on their ability to induce ADCC or ADCP in patients. Our ADCC and ADCP assays are based on iLite® technology, a cleverly engineered cell-based system with a dual reporter gene readout. They offer a convenient and powerful way of measuring the efficacy of antibodies to elicit ADCC or ADCP in vitro.

iLite ADCC & ADCP Reporter Gene Assays

iLite Fc Effector Activity Bioassays are reporter gene cell lines and antigen-specific target cells designed to measure the Fc effect of antibodies and other biologics. These bioassays utilize a luciferase reporter gene system to conveniently assess the efficacy of antibodies in eliciting ADCC and ADCP in vitro.

  • Biologically relevant MoA-reflective assays
  • Unparalleled sensitivity for each target
  • A rapid and convenient workflow with reduced assay variability
  • Assays suitable for antibody screening, characterization, stability, and potency studies
  • A secondary reporter gene that acts as an internal control, compensating for differences in cell number and matrix effects
  • "Assay-ready" format for rapid and convenient use
  • Effector and Target cells engineered to work optimally together

Our ADCC & ADCP Reporter-Gene Assays

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ALL ADCC ADCP Effector Cells Target cells
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BM5001

iLite® ADCC Effector FcγRIIIa (V)

Designed to quantify ADCC activity

CELL-BASED
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Cell-based reporter gene bioassay | Fc Effector Cells | BM5001 | ADCC | Cell-based reporter gene assay | Luminescence |
BM5004

iLite® ADCP Effector FcγRIIa

Accurate measurement of ADCP activity

CELL-BASED
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Cell-based reporter gene bioassay | Fc Effector Cells | BM5004 | ADCP | Cell-based reporter gene assay | Luminescence |
BM5025

iLite® CD19 (+) Target

Engineered to express CD19, this cell line is ideal for ADCC and bispecific antibody assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5025 | ADCC | T-Cell Activation | Cell-based reporter gene assay | Luminescence |
BM5026

iLite® CD19 (-) Target

CD19-negative target cell line used as a specificity control in ADCC and T cell engager assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5026 | ADCC | T-Cell Activation | Cell-based reporter gene assay | Luminescence |
BM5010

iLite® CD20 (+) Target

Target cells expressing CD20 for use in ADCC and ADCP bioassays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5010 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5015

iLite® CD20 (-) Target

CD20-negative target cells. Ideal for use as controls in CD20-specific bioactivity assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5015 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5035

iLite® EGFR (+) Target

Well-suited for ADCC or bispecific antibody assays targeting EGFR-positive cells

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5035 | ADCC | Cell-based reporter gene assay | Luminescence |
BM5036

iLite® EGFR (-) Target

A negative control cell line for EGFR-targeted bioassays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5036 | ADCC | Cell-based reporter gene assay | Luminescence |
BM5011

iLite® HER2 (+) Target

Target cells for functional antibody testing in HER2-targeted bioassays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5011 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5016

iLite® HER2 (-) Target

HER2-negative target cell line used as a control in HER2 bioactivity, ADCP, and ADCC assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5016 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5013

iLite® mTNF-alpha (+) Target

Target cells for functional characterization of TNF-targeting biologics

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5013 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5014

iLite® mTNF-alpha (-) Target

A control cell line used for specificity assessment in TNF-targeted assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5014 | ADCC | ADCP | Cell-based reporter gene assay | Luminescence |
BM5017

iLite® mVEGF (+) Target

Target cells for potency assays of anti-VEGF agents

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5017 | ADCC | Cell-based reporter gene assay | Luminescence |
BM5018

iLite® mVEGF (-) Target

Negative control cell line for VEGF-targeted assays

CELL-BASED
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Cell-based reporter gene bioassay | Target Cells | BM5018 | ADCC | Cell-based reporter gene assay | Luminescence |

Didn't find what you were looking for?

We offer products customized to your needs. Our custom iLite® reporter-gene assays can be adapted to almost any target. We also offer custom ELISA plates and other products. In addition, you can take advantage of our service offerings and let us do the work for you.

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iLite News

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Introducing the new iLite® C3a Assay Ready Cells

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Improved Speed and Accuracy in Immunogenicity Testing

Improved Speed and Accuracy in Immunogenicity Testing

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Transitioning to Second-Generation iLite Cells

Transitioning to Second-Generation iLite Cells

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iLite Downloads

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IL-6 Biology, Inhibitors & COVID-19

IL-6 Biology, Inhibitors & COVID-19

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Mechanism-linked Potency Assays Drive Gene Therapy Success

Mechanism-linked Potency Assays Drive Gene Therapy Success

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Addressing the Analytical Gaps in Modern Therapeutic Development

Addressing the Analytical Gaps in Modern Therapeutic Development

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Developing a Customizable Complement-Dependent Cytotoxicity Assay

Developing a Customizable Complement-Dependent Cytotoxicity Assay

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Partnering for Success in a New Era of Advanced Therapeutics

Partnering for Success in a New Era of Advanced Therapeutics

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The Immune Response to AAV Gene Therapy

The Immune Response to AAV Gene Therapy

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How to Design Potency Assays for Gene Therapy Projects

How to Design Potency Assays for Gene Therapy Projects

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A Cell-Based ADCC Bioassay Comparison

A Cell-Based ADCC Bioassay Comparison

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Quantification of the Neutralizing Antibody Response to Recombinant AAV Vectors

Quantification of the Neutralizing Antibody Response to Recombinant AAV Vectors

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Svar Complement Assays: Analyzing How Drug Candidates Affect the Complement System

Svar Complement Assays: Analyzing How Drug Candidates Affect the Complement System

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Assay Development in AAV Therapies: Engineering iLite® Cell Lines for Specific and Analytical Applications

Assay Development in AAV Therapies: Engineering iLite® Cell Lines for Specific and Analytical Applic...

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Measuring C5a With a Functional Bioassay

Measuring C5a With a Functional Bioassay

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Establishment of a Novel iLite® Reporter Gene Assay Response to Insulin Products

Establishment of a Novel iLite® Reporter Gene Assay Response to Insulin Products

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Evaluation of iLite® ADCC Effector Cells Using Different CD20 Target Cells

Evaluation of iLite® ADCC Effector Cells Using Different CD20 Target Cells

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Development of an FGF21 iLite® Reporter Gene Cell Line

Development of an FGF21 iLite® Reporter Gene Cell Line

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