iLite® TNF-alpha Xcel Assay Ready Cells
BM4044
The iLite® TNF-alpha Xcel Assay Ready Cells provide a means of directly quantifying both the potency and neutralizing antibody response to TNF-alpha antagonists of diverse structure in the same assay, making it an outstanding tool for biosimilar development.
Measuring the functional activity of TNF-alpha, the assay can be used for both potency and immunogenicity assessments of TNF-alpha inhibitors in the same assay and the iLite TNF-alpha Xcel Assay Ready Cells also provide a means of directly comparing a biosimilar and innovator product in the same assay.
The iLite TNF-alpha cells are engineered cells for use in a normalized reporter gene bioassay for screening of anti-TNF alpha activity in samples. The assay is a simple and easy test format which detects anti-TNF alpha activity and, indirectly, the presence of anti-drug antibodies.
The iLite TNF-alpha Xcel Assay Ready Cells are engineered cells, optimized to be responsive to TNF-alpha, resulting in a proportional expression of Firefly Luciferase. Binding of TNF-alpha to its receptor results in activation of the NFkB regulated Firefly luciferase reporter gene construct. iLite TNF-alpha Xcel Assay Ready Cells also contain the Renilla Luciferase reporter gene, under the control of a constitutive promoter.
FOR RESEARCH USE ONLY
At a glance
| Product code | BM4044 |
| Assay target | TNF-alpha |
| Size | 1 vial corresponding to one 96 well plate |
| Read-out of results | Semi-quantitative, |
| Assay time | 3,5-4h |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
What's in the box?
1.5 mL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), 10% glycerol and 2.5% dimethyl sulfoxide (DMSO).
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