DIAGNOSTIC TEST 245

PLA2R Antibodies IIF (IgG)

Diagnostic test for antibodies against PLA2R. For suspicion of primary membranous nephropathy (PMN).

swedac

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Indication

Suspicion of primary membranous nephropathy (PMN).

Sample material

Serum

  • Minim. volume: 0,5 mL

Transport

Within Sweden

  • room temperature

International

  • cold

Method

Cell-based assay (CBA), Indirect immunofluorescence(IIF)

Reference interval

<1:10 negative

Result

Results are reported as negative or positive. Positive results will be quantified with ELISA (RU/mL).

Interpretation

Antibodies against PLA2R are a strong marker for primary membrane nephropathy (PMN). Antibodies against PLA2R lead to complement-activating immune complexes deposited on the basal membrane in glomeruli, with tissue damage and proteinuria as a consequence.

Antibodies against PLA2R occur in serum in 75% of the PMN cases. The concentration of antibodies is associated with disease development, and their concentration determination by ELISA (RU/ml) can be used to follow the treatment effect.

In case of negative results, it is recommended to supplement with testing for antibodies against Thrombospondin Type 1 Domain Containing 7A (THSD7A) which occurs in approximately 10% of anti-PLA2R negative PMN. The simultaneous presence of antibodies against PLA2R and THSD7A is very unusual.

References

  • Beck LH Jr et al. N Engl J Med. 2009. M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy. PMID: 19571279
  • Ronco P, Debiec H. J Am Soc Nephrol. 2010. Antigen identification in membranous nephropathy moves toward targeted monitoring and new therapy. PMID: 20185638
  • Gödel M et al. N Engl J Med. 2015. Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy. PMID: 25760365
  • Tomas NM et al. N Engl J Med. 2014. Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy. PMID: 25394321
  • Ronco P, Debiec H. Lancet. 2015. Pathophysiological advances in membranous nephropathy: time for a shift in patient's care. PMID: 26090644
  • Larsen CP et al. Mod Pathol. 2016. THSD7A staining of membranous glomerulopathy in clinical practice reveals cases with dual autoantibody positivity. PMID: 26847174

Included in these panels

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How to order

This test is available worldwide for hospitals, clinics, and physicians.

  1. Print and complete the request form

    Download the request form. Clearly state the name and phone number of the referring hospital, clinic, or physician.

  2. Prepare your samples

    Serum: At least 0.5 mL serum (plain serum tubes without additives).

  3. Send samples and request form

    Within Sweden
    Samples can be sent at room temperature to:
    Envelopes and smaller boxes:
    Wieslab AB, Box 50117, 20211 Malmö, Sweden

    Larger boxes and frozen samples:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

    International
    Send samples cold to:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

Read our sampling instructions for more information

Last updated: 2025-08-18