Development of an FGF21 iLite^® Reporter Gene Cell Line

Scientific poster title: "Development of an iLite^® Reporter Gene Cell Line for the Quantification of the Potency of Analogues of Human FGF21"

In this scientific poster, we showcase that normalization of drug-induced reporter gene activity, relative to the expression of an internal standardization gene under the control of a constitutive promoter, renders assay results independent of cell number and also provides a means for correcting for serum matrix effects and allows cytokine levels to be quantified even in sera with a relatively high level of cytotoxicity. 

The iLite technology can be adapted for many applications throughout the drug development phases including drug manufacturing processes such as the quantification of vector potency and functionality or the detection of neutralizing anti-drug antibodies (NAb).