DIAGNOSTIC TEST 008

C-/P-ANCA

Diagnostic test for suspicion of systemic vasculitis and inflammatory diseases.

Indication

Suspicion of systemic vasculitis and inflammatory diseases

Sample material

Serum

  • Minim. volume: 0,5 mL

Transport

  • Within Sweden: room temperature
  • International: cold

Method

Indirect immunofluorescence (IIF)

Reference interval

<1:10 negative

Result

Results are reported as negative or positive with a titer.

Interpretation

C-ANCA is often caused by antibodies against proteinase-3 (PR3), a marker for the vasculitis disease granulomatosis with polyangiitis (GPA). In the first place, specific analyses of PR3-ANCA and MPO-ANCA are recommended if ANCA-associated vasculitis is suspected.

P-ANCA with specificity for myeloperoxidase (MPO) is a marker for the vasculitis microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (EGPA), but can also be found without associated vasculitis. Initially, specific analyses of PR3-ANCA and MPO-ANCA are recommended if ANCA-associated vasculitis is suspected.

P-ANCA with other specificity (for example, elastase or lactoferrin) can be seen in multiple different conditions, for example, inflammatory bowel disease, but has low clinical value.

MPO- and PR3-ANCA can occasionally give an atypical ANCA-pattern and determination of these specificities is recommended if ANCA-associated vasculitis is suspected. Atypical ANCA due to other antibody specificities has low clinical value.

References

  • Suppiah R et al. Arthritis Rheumatol. 2022. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology Classification Criteria for Microscopic Polyangiitis. PMID: 35106973
  • Grayson PC et al. Arthritis Rheumatol. 2022. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology Classification Criteria for Eosinophilic Granulomatosis With Polyangiitis. PMID: 35106968
  • Grayson PC et al. Arthritis Rheumatol. 2022. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology Classification Criteria for Eosinophilic Granulomatosis With Polyangiitis. PMID: 35106968
  • Rathmann J et al. Rheumatology (Oxford). 2024. Evaluation of the ACR/EULAR 2022 criteria for classification of ANCA-associated vasculitis in a population-based cohort from Sweden. PMID: 37758240
  • Guchelaar NAD et al. Autoimmun Rev. 2021. The value of anti-neutrophil cytoplasmic antibodies (ANCA) testing for the diagnosis of ANCA-associated vasculitis, a systematic review and meta-analysis. PMID: 33197574

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How to order

This test is available worldwide for hospitals, clinics, and physicians.

  1. Print and complete the request form

    Download the request form. Clearly state the name and phone number of the referring hospital, clinic, or physician.

  2. Prepare your samples

    Serum: At least 0.5 mL serum (plain serum tubes without additives).

  3. Send samples and request form

    Within Sweden
    Samples can be sent at room temperature to:
    Envelopes and smaller boxes:
    Wieslab AB, Box 50117, 20211 Malmö, Sweden

    Larger boxes and frozen samples:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

    International
    Send samples cold to:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

Read our sampling instructions for more information

Last updated: 2025-12-10