DIAGNOSTIC TEST 023

dsDNA Antibodies (IgG)

Diagnostic test for antibodies against dsDNA. For suspicion of systemic lupus erythematosus (SLE) or incipient exacerbation of this disease, autoimmune hepatitis type.

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Indication

Suspicion of systemic lupus erythematosus(SLE) or incipient exacerbation of this disease, chronic aggressive hepatitis of the “lupoid hepatitis” (autoimmune hepatitis) type.

Sample material

Serum

  • Minim. volume: 0,5 mL

Transport

Within Sweden

  • room temperature

International

  • cold

Method

Indirect immunofluorescence (IIF) on Crithidia luciliae

Reference interval

<1:10 negative

Result

Results are reported as negative or positive with a fluorescence intensity. Positive results will be titrated.

Interpretation

IgG antibodies against dsDNA are a strong marker for systemic lupus erythematosus (SLE) and are associated with SLE-nephritis but can also be seen in autoimmune hepatitis type 1. In SLE, a rise in antibody level often indicates increased disease activity, while levels often drop under remission.

References

  • Enocsson H et al. J Rheumatol. 2015. Four Anti-dsDNA Antibody Assays in Relation to Systemic Lupus Erythematosus Disease Specificity and Activity. PMID: 25684763
  • Wiik A et al. Lupus. 2006. European attempts to set guidelines for improving diagnostics of autoimmune rheumatic disorders.;15(7):391-6. doi: 10.1191/0961203306lu2322oa. PMID: 16898171
  • Yee CS et al. Arthritis Rheum. 2007. British Isles Lupus Assessment Group 2004 index is valid for assessment of disease activity in systemic lupus erythematosus. PMID: 18050213
  • Hamann D, Smeenk RJT. dsDNA autoantibodies. In: Shoenfeld Y, Gershwin ME, Meroni PL, editors. Autoantibodies. London: Elsevier; 2006. p. 159-67.
  • Ho A et al. Arthritis Rheum. 2001. Decreases in anti-double-stranded DNA levels are associated with concurrent flares in patients with systemic lupus erythematosus. PMID: 11665975
  • Swaak T, Smeenk R. Ann Rheum Dis. 1985. Detection of anti-dsDNA as a diagnostic tool: a prospective study in 441 non-systemic lupus erythematosus patients with anti-dsDNA antibody (anti-dsDNA). PMID: 3872637
  • Anti-Nuclear Factor Serum (Homogeneous) Human NIBSC code: 66/233 Instructions for use (Version 6.0, Dated 11/04/2008)

Included in these panels

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How to order

This test is available worldwide for hospitals, clinics, and physicians.

  1. Print and complete the request form

    Download the request form. Clearly state the name and phone number of the referring hospital, clinic, or physician.

  2. Prepare your samples

    Serum: At least 0.5 mL serum (plain serum tubes without additives).

  3. Send samples and request form

    Within Sweden
    Samples can be sent at room temperature to:
    Envelopes and smaller boxes:
    Wieslab AB, Box 50117, 20211 Malmö, Sweden

    Larger boxes and frozen samples:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

    International
    Samples can be sent at room temperature to:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

Read our sampling instructions for more information

Last updated: 2025-08-18