DIAGNOSTIC TEST 200

Neuronal Antibody Screen – Tissue-Based Assay (TBA)

Diagnostic test for antibodies against neuronal antigens. For suspicion of autoimmune neuroinflammation.

Indication

Suspicion of autoimmune neuroinflammation

Sample material

Serum

  • Minim. volume: 0,5 mL

CSF

  • Minim. volume: 1,5 mL

Transport

  • Within Sweden: room temperature
  • International: cold

Method

Tissue-based assay (TBA), indirect immunofluorescence microscopy on hippocampus and cerebellum (murine).

Reference interval

Serum: <1/10 negative

CSF: negative

Result

Results are reported as negative or as a finding that indicates presence of antibodies. These findings are verified with antigen specific methods.

Interpretation

Screening of antibodies against neuronal antigens analyzed by immunofluorescence microscopy on sectioned murine brain tissue. Antibodies against neuronal antigens are detected as fluorescens patterns dependent on the antigen’s distribution in the tissue.

The test could be requested as initial screening investigation. If a pattern specific to an antibody is detected, the specificity is verified by antigen specific method.

The test can also be requested for samples that initially have been analyzed with antigen specific tests:

  1. The test is recommended when antibody mediated neuroinflammation is suspected. Positive findings are verified with an antigen-specific method.
  2. The test is also recommended as a complement for samples with negative results on antigen specific tests, since more antigens are exposed on neuronal tissue.

References

  • Jarius S, Wildemann B. J Neuroinflammation. 2015. 'Medusa-head ataxia': the expanding spectrum of Purkinje cell antibodies in autoimmune cerebellar ataxia. Part 1: Anti-mGluR1, anti-Homer-3, anti-Sj/ITPR1 and anti-CARP VIII. PMID: 26377085
  • Jarius S, Wildemann B. J Neuroinflammation. 2015. 'Medusa head ataxia': the expanding spectrum of Purkinje cell antibodies in autoimmune cerebellar ataxia. Part 2: Anti-PKC-gamma, anti-GluR-delta2, anti-Ca/ARHGAP26 and anti-VGCC. PMID: 26377184
  • Goncalves D et al. Front Immunol. 2025. Comparison of commercial and in-house tissue-based and cell-based assays for the detection of autoantibodies targeting neuronal surface proteins: a prospective cohort study. PMID: 40303411
  • Milano C et al. Neurol Neuroimmunol Neuroinflamm. 2025. Assessing Commercial Tissue-Based Assays for Autoimmune Neurologic Disorders (I): Antibodies to Intracellular Antigens. PMID: 40393022
  • Papi C et al. Neurol Neuroimmunol Neuroinflamm. 2025. Assessing Commercial Tissue-Based Assays for Autoimmune Neurologic Disorders (II): Antibodies to Surface Antigens. PMID: 40393020
  • Landa J et al. Ann Neurol. 2021. Encephalitis with Autoantibodies against the Glutamate Kainate Receptors GluK2. PMID: 33949707

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How to order

This test is available worldwide for hospitals, clinics, and physicians.

  1. Print and complete the request form

    Download the request form. Clearly state the name and phone number of the referring hospital, clinic, or physician.

  2. Prepare your samples

    Serum: At least 0.5 mL serum (plain serum tubes without additives).
    CSF: At least 1.5 mL CSF (polypropylene tubes).

  3. Send samples and request form

    Within Sweden
    Samples can be sent at room temperature to:
    Envelopes and smaller boxes:
    Wieslab AB, Box 50117, 20211 Malmö, Sweden

    Larger boxes and frozen samples:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

    International
    Send samples cold to:
    Wieslab AB, Lundavägen 151, 21224 Malmö, Sweden

Read our sampling instructions for more information

Last updated: 2026-01-29