iLite® GLP-1 Assay Ready Cells
BM4100
This iLite® GLP-1 reporter assay provides cell-based functional studies of GLP-1 and its receptor GLP1R, supporting metabolic research and development of incretin-based single- and multi-agonist therapeutics.
- High sensitivity and large dynamic range
- "Assay ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® GLP-1 Assay Ready Cells are based on the human embryonic kidney cell line, HEK293, and have been genetically engineered and optimized to be responsive to GLP-1 receptor agonists, resulting in a proportional expression of Firefly Luciferase.
The iLite GLP-1 Assay Ready Cells can be used for functional studies of GLP-1, the receptor GLP1R, and their interaction in test samples, including human serum.
Normalization of cell counts and serum matrix effects is achieved using a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promoter.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of iLite Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM4100 |
| Assay target | GLP-1 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
| Assay time | 5h |
Documents
Application Note
