iLite® GIP Assay Ready Cells
BM4110
This iLite® GIP reporter assay provides cell-based functional studies of GIP and its receptor GIPR, supporting metabolic research and development of incretin-based therapeutics.
- High sensitivity and large dynamic range
- "Assay ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® GIP Assay Ready Cells are based on the human embryonic kidney cell line, HEK293, and have been genetically engineered and optimized to be responsive to GIP receptor agonists, resulting in a proportional expression of Firefly Luciferase.
The iLite GIP Assay Ready Cells can be used for functional studies of GIP receptor agonists and for the detection of GIP inhibitor activity or GIP receptor inhibitor activity in test samples, including human serum.
Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promoter.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM4110 |
| Assay target | GIP |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
| Assay time | 5h |
Documents
Application Note
Safety Data Sheet
