iLite® Insulin Assay Ready Cells

BM3060

This iLite® insulin assay offers robust, quantitative measurement of insulin receptor activation, facilitating characterization of insulin analogs and biosimilars.

  • High sensitivity and large dynamic range
  • "Assay-ready" format for rapid and convenient use
  • Reproducible and biologically relevant results
  • Reduced assay variability and higher reproducibility compared to cells in culture
  • Normalization of cell counts and serum matrix effects using a second reporter gene
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iLite® Insulin Assay Ready Cells

Product Overview

The insulin-responsive iLite® Insulin Assay Ready Cells are avian DT-40 cells (ATCC#CRL2111) genetically engineered to be responsive to Insulin, resulting in the specific expression of Firefly Luciferase. They provide the basis for the establishment of a rapid, sensitive, and highly specific assay for the quantification of insulin activity that can be completed within 6 hours.

The assay can be used to quantify the activity of insulin or an insulin analog in human serum without interference from factors present in human serum, including IGF-1. In addition, iLite assay ready cells can be used for measuring the presence of neutralizing anti-insulin antibodies.

Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.

 

The ATCC® CRL-2111 cell line is used under a license from ATCC®

FOR RESEARCH USE ONLY

How it works

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The function of iLite assays

iLite cells have receptors on the cell surface that can bind to the marker under study.

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The function of iLite assays

Upon binding of the marker to its receptor, a signaling path activates the production of luciferase from a reporter gene.

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The function of iLite assays

Following cell lysis and the addition of a substrate, the luciferase is measured using a luminometer.

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The function of iLite assays

iLite cells contain a secondary Renilla luciferase gene that is constitutively expressed and is used for normalization.

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What's in the box?

>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).

Specifications

Product code BM3060
Assay target Insulin
Size 1 vial corresponding to one 96 well plate
Technology Cell-based reporter gene assay
Detection system Luminescence
Regulatory status Research Use Only
Assay time 5,5-6h

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