iLite® CD20 (+) Svar Luc Assay Ready Cells
BM5028
iLite® CD20 (+) Svar Luc Assay Ready Cells were designed for sensitive and reproducible studies of complement-dependent cytotoxicity (CDC). They enable robust assessment of CDC activity, antibody screening, and functional inhibition using luciferase-based readout.
- "Assay-ready" format for rapid and convenient use
- Reproducible and safe
- A true measure of cell lysis
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
The iLite® CD20 (+) Svar Luc Assay Ready Cells are used for studies of complement-dependent cytotoxicity in test samples, including human serum, to investigate the activity of complement-dependent cytotoxicity in different samples. The cells can also be used to assess the efficiency and screening of antibodies of interest in the development of therapeutic antibodies. Moreover, cells can be utilized to quantify the inhibition of complement-dependent cytotoxicity activity.
iLite® CD20 (+) Svar Luc Assay Ready Cells are human B lymphocyte Ramos cells (ATCC #CRL-1596) that have been genetically engineered to express Svar luciferase constitutively.
FOR RESEARCH USE ONLY
How it works
Principle of the CDC bioassay
Our CDC cell lines express Svar luciferase from a constitutive promoter. In addition, they overexpress antigens on the cell surface that can be bound by a therapeutic drug antibody.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11893)
Specifications
| Product code | BM5028 |
| Assay target | CDC |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
Safety Data Sheet
