iLite® AAV8 Packaging Assay Ready Cells

BM6008

iLite® AAV Packaging Assay Ready Cells are ideal for neutralizing antibody detection studies. Engineered and optimized to produce AAV vectors of serotype 8.

  • "Assay-ready" format for rapid and convenient use
  • Reproducible and biologically relevant results
  • Reduced assay variability and higher reproducibility compared to cells in culture
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iLite® AAV8 Packaging Assay Ready Cells

Product Overview

iLite® AAV8 Packaging Assay Ready Cells are based on the human embryonic kidney 293 (HEK293) cell line. They are ideal for neutralizing antibody detection studies. Engineered and optimized to produce AAV vectors of serotype 8.

How it works

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AAV packaging cell

A two-cell line setup

A packaging cell generates AAVs. We offer many different kinds of packaging cells, each producing a different AAV serotype.

AAV iLite cell

A two-cell line setup

An AAV-responsive reporter cell expresses Firefly luciferase in response to AAV transduction that can be measured using a standard luminometer. In the presence of neutralizing antibodies, the AAV transduction is impaired, resulting in a lower signal. A constitutively expressed second Renilla luciferase reporter gene can be used as an internal control of matrix effects.

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What's in the box?

>250 µL of Assay Ready Cells suspended in recovery medium from Gibco (Cat. No 12648-010).

Specifications

Product code BM6008
Assay target AAV
Technology Cell-based reporter gene assay
Detection system Luminescence
Regulatory status Research Use Only