In contrast to hemolytic assays, the Svar Complement functional ELISA is based on quantifying the formation of the Membrane Attack Complex, and will reflect all the different steps in the complement activation pathways and hence provide the full picture of complement function.
Compared to traditional hemolytic assays (CH50, AH50) the Svar Complement functional assays offer increased reagent stability and less analytical variation, thereby strengthening and improving functional assessment of complement in different analytical situations.
The Svar functional assay allow for measurement of the lectin pathway, and properdin deficiency is detectable with our alternative pathway, which is not possible with hemolytic methods.
The ELISA format used offers superior ease of handling, increased objectivity in interpretation and faster turnaround time. The Svar assays are also optimized for automation resulting in decreased hands-on-time and increased reliability and reproducibility.
In a number of situations, it is assumed that the concentrations of certain complement components (C3 and C4) reflect the overall functionality and activity of the complement system. Only measuring the level of C3 and C4 provides an oversimplified and incomplete view, and is associated with serious limitations such as: