Olink® Technology - Proximity Extension Assay (PEA) technology

Innovation for protein biomarker discovery & protein analysis.

Proximity Extension Assay (PEA) technology is the unique technology behind Olink® Signature platform, Olink® Target 96 and Target 48 panels. 

It enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample. The technology delivers this scale of multiplexing and sample throughput without compromising data quality or assay robustness.

Overcoming the specificity issues of multiplexed immunoassays

Each of the 96 oligonucleotide antibody pairs contains unique DNA sequences allowing hybridization only to each other. Subsequent proximity extension will create 96 unique DNA reporter sequences amplified by real-time PCR. A limiting factor of multiplexed immunoassays is the antibody cross-reactivity which restricts the degree of multiplexing of most assays to below 10-plex. Olink’s panels will not detect cross-reactive events since only matched DNA reporter pairs can hybridize to produce an amplicon for NGS or real-time qPCR. This allows for scalable multiplexing without loss of specificity and sensitivity.

View more information on olink.com/our-platform/our-pea-technology


The unique technology behind the Olink platforms, Proximity Extension Assay (PEA) technology, is an innovative dual recognition, DNA-coupled methodology providing exceptional readout specificity. PEA enables high multiplex, rapid throughput biomarker analysis without compromising on data quality.

Antibody pairs labelled with DNA oligonucleotides bind target antigen in solution, allowing hybridization and extension by DNA polymerase. This newly created piece of DNA barcode is amplified by standard PCR before transfer to an integrated microfluidic chip (IFC), which is loaded into the instrument for qPCR and data readout.

Signature — an integrated protein analysis solution

Olink® Signature Q100 is a desktop instrument that allows seamless Olink® PEA protein analysis to get biological insights. High-quality protein data is obtained using scalable and flexible multiplex protein panels that require only one microliter of blood for analysis.

Streamlined - both in form and function

Olink Signature Q100 is streamlined in both form and function. Based on the Fluidigm Biomark HD, it utilizes the advantages of nanoliter scale microfluidics to conserve precious samples and reagents and decreases time to result through workflow automation.

Advantages of the Signature Q100 instrument:

  • A compact benchtop design
  • Integration of IFC loader, thermal cycler, and operating software in one machine controlled through a smart, interactive touchscreen.
  • Easy to use; carry out all aspects of your analysis from sample preparation to qPCR to data download using one instrument interface.
Olink Signature Q100 supports the analysis of all Olink® Target protein panels. Each of these panels has been designed to give you a broad understanding of a particular disease or biological process, and the library includes a comprehensive collection of inflammation protein biomarkers that can be applied to a wide range of diseases. Depending on your protein analysis needs, you can easily change between different panels and formats and scale up or down.

Workflow with Olink® Signature Q100: Signature Q100 has been created with a seamless workflow in mind, combining sample and IFC loading,qPCR readout, data pre-processing, and data analysis into one single instrument and software