Svar Life Science
Get a custom iLite cell line for your target of choice

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Cell Line Development

Get a custom iLite® cell line tailored to your needs

iLite® technology is a powerful cell-based technique used to measure and quantify molecules in biological samples. It has applications throughout the drug development process as well as for monitoring biological drugs.

Svar Life Science offers iLite cells for a long list of targets. However, should your target not be included on the list, we can create a custom iLite cell line tailored for your needs. In addition, we offer a wide range of custom services to support drug development programs from discovery to lot release.
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Creating a custom iLite cell line

We have over 15 years of experience creating iLite cell lines. In addition to our off-the-shelf products, we have developed custom assays for a wide range of targets and customers.

"Our in-house expertise and processes have led to a success rate of 100%.
We can create an iLite cell line for almost any biopharmaceutical target."
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How to get your custom iLite cell line – Outline of a project

1. Project definition

  • Consultation – We discuss and agree on project goals
  • Research – Our Paris cell assay team investigates feasibility and develops an execution plan
  • Scope – Get a complete project proposal outlining the scope of work and a master service agreement

2. Project execution

  • Bi-weekly or monthly tele-conference updates
  • Generation of stable transfection
  • Sub-cloning
  • Creation of a master cell bank
  • Creation of a working cell bank
  • Define cell replication step
  • Stability testing
  • Cryo-preservation
  • Manufacturing of assay-ready cells

3. Delivery

  • Delivery of a qualified, stable custom iLite cell line
  • Validation of the cell line (optional)

Total project time: 6-9 months

The benefits of iLite technology

Each iLite cell has receptors on its cell surface which are specific for the molecule for which the cell line was designed. Upon binding of the molecule to its receptor, a luciferase reporter gene is activated. Following cell lysis and the addition of a substrate, the luciferase protein can be measured using a luminometer.

As a unique feature, iLite cells contain a second reporter gene which is used for normalization purposes. It can be used to compensate for serum matrix effects or for differences in cell number for example.

iLite cells are shipped in an assay-ready format, which offers great convenience and improved reproducibility while at the same time saves time and labor.

 

Want a custom iLite cell line?

Contact us to learn more about this offer and find out how we can help.

 

Contact us