Svar Life Science

March 7, 2023

Properdin functional and quantitative assays: a new bi-dimensional tool boosting the next generation of complement therapeutics

Introducing Factor P (Properdin) quantitative and functional assay - for better resolution of the amplification loop.

The new Factor P quantitative and functional assays offer a flexible and complete solution to study properdin.

The Svar solution is a bi-dimensional tool that consists of: 

  • A high-performing quantitative ELISA for human Factor P that ensures the detection in serum and plasma, giving results in just 2 hours
  • A functional assay that utilizes the same, high-performing Factor P capture antibody as the quantitative assay and a detection antibody targeting the downstream products of the stabilized C3 convertase to assess the Properdin function.  

March 7, 2023

Properdin functional and quantitative assays: a new bi-dimensional tool boosting the next generation of complement therapeutics

Introducing Factor P (Properdin) quantitative and functional assay - for better resolution of the amplification loop.

The new Factor P quantitative and functional assays offer a flexible and complete solution to study properdin.

The Svar solution is a bi-dimensional tool that consists of: 

  • A high-performing quantitative ELISA for human Factor P that ensures the detection in serum and plasma, giving results in just 2 hours
  • A functional assay that utilizes the same, high-performing Factor P capture antibody as the quantitative assay and a detection antibody targeting the downstream products of the stabilized C3 convertase to assess the Properdin function.  

WHAT IS FACTOR P?

Properdin is an innate modifier of complement. It performs several functions which contribute to up regulation of the complement response, inhibiting down regulators’ ability to bind and cleave functional components of the cascade, as well as stabilizing the C3 and C5 convertases of the AP, decreasing their disassociation rates.

Properdin is mainly synthesized by leucocytes, in contrast to the majority of complement proteins, which are synthesized in the liver. In response to inflammatory triggers, it is quickly released and stabilizes the assembly of C3 and C5 convertases.

Read more about Properdin and Factor P assays here:

Factor P - Properdin assays

Complement-roadmap-properdin-highlights

Did you know the components of the amplification loop are novel key targets of emerging complement therapeutics?

Complement therapeutics focusing on the amplification loop have the potential to improve disease outcomes and preserve the protective capabilities of the complement system2-4.

An increasingly popular approach is identifying novel targets to modulate the complement pathways' response rather than completely inhibiting their activity2,3.  The amplification loop - which has often been overlooked in complement therapeutics development - offers a great potential for drug development, as reflected by the current landscape of emerging drugs targeting the alternative pathway to treat a wide range of diseases. 


WHY LOOK AT THE AMPLIFICATION LOOP?

The amplification loop is an important contributor to overall complement cascade activity and plays a crucial role in amplifying any small trigger to a bigger downstream response1. Low-level complement activity is constantly ongoing, especially in the autohydrolysis of the alternative pathway (AP), and the amplifying loop allows mounting a quick response. 

The amplification loop comprises a range of components, like Factor B and Factor D, and modulators that are crucial for its functioning and its ability to regulate the overall cascade.

These include inhibitors such as DAF (CD55), Factor H, and  Factor I, as well as the only known up regulator, properdin (Factor P), that serves as an amplifier of the alternative pathway, thus making this loop a crucial element in the control of the overall cascade.  

 

 


Find the full range of our Complement assay: 

Functional Assays

Biomarker Assays 

Cell-based Assays

GET THE BEST OUT OF FACTOR P, THE PIONEERING PROPERDIN FUNCTIONAL ASSAY

Factor P functional and quantitative assays, the latest addition to our Complement product range!

Together the quantitative and functional features give you the possibility that, apart from measuring the levels of properdin and determine its functionality, to easily adapted for more in depth studies, granting a closer look at the amplification loop!

 Benefit from our expertise and innovative solutions  - talk to us about your Factor P testing needs today!

    Contact us!
References:

  1. Harboe, M. & Mollnes, T. E. The alternative complement pathway revisited. Journal of Cellular and Molecular Medicine 12, 1074–1084 (2008).

  2. Harris, C. L. Expanding horizons in complement drug discovery: challenges and emerging strategies. Semin Immunopathol 40, 125–140 (2018).

  3.  Zelek, W. M., Xie, L., Morgan, B. P. & Harris, C. L. Compendium of current complement therapeutics. Molecular Immunology 114, 341–352 (2019).

  4.  McNamara, L. A. et al. High Risk for Invasive Meningococcal Disease Among Patients Receiving Eculizumab (Soliris) Despite Receipt of Meningococcal Vaccine. 66, (2017).

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