iLite® TNF-alpha


The iLite® TNF-alpha Assay Ready Cells provide a means of directly quantifying both the potency and neutralizing antibody response to TNF-alpha antagonists of diverse structure in the same assay, making it an outstanding tool for biosimilar development.

Measuring the functional activity of TNF-alpha, the assay can be used for both potency and immunogenicity assessments of TNF-alpha inhibitors in the same assay and the iLite TNF-alpha assay ready cells also provide a means of directly comparing a biosimilar and innovator product in the same assay.

The iLite TNF-alpha cells are engineered cells for use in a normalized reporter gene bioassay for screening of anti-TNF alpha activity in samples. The assay is a simple and easy test format which detects anti-TNF alpha activity and, indirectly, the presence of anti-drug antibodies.

iLite TNF-alpha cells are a genetically engineered reporter gene cell line of human origin (K562), responsive to tumor necrosis factor alpha (TNF-alpha), resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.


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iLite®  TNF-alpha
iLite®  TNF-alpha

At a glance

Product code BM3044
Assay target TNF-alpha
Size 1 vial corresponding to one 96 well plate
Read-out of results Semi-quantitative,
Assay time 3,5-4h
Detection system Luminescence
Regulatory status Research Use Only

What's in the box?

1.5 mL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), 10% glycerol and 2.5% dimethyl sulfoxide (DMSO).


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