iLite® TNF-alpha Xcel Assay Ready Cells
BM4044
Pre-qualified and assay-ready, iLite® TNF-α Xcel Cells provide rapid and sensitive detection of TNF-α activity – streamlining workflows for potency testing and neutralization assays.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® TNF-alpha Xcel Assay Ready Cells provide a means of directly quantifying both the potency and neutralizing antibody response to TNF-alpha antagonists of diverse structure in the same assay, making it an outstanding tool for biosimilar development. The assay is a simple and easy test format which detects anti-TNF alpha activity and, indirectly, the presence of anti-drug antibodies.
Measuring the functional activity of TNF-alpha, the assay can be used for both potency and immunogenicity assessments of TNF-alpha inhibitors in the same assay and the iLite TNF-alpha Xcel Assay Ready Cells also provide a means of directly comparing a biosimilar and innovator product in the same assay.
iLite® TNF-alpha Xcel Assay Ready Cellsare engineered cells for use in a normalized reporter gene bioassay for screening of anti-TNF alpha activity in samples, resulting in a proportional expression of Firefly Luciferase. Binding of TNF-alpha to its receptor results in activation of the NFkB regulated Firefly luciferase reporter gene construct. iLite® TNF-alpha Xcel Assay Ready Cells also contain the Renilla Luciferase reporter gene, under the control of a constitutive promoter.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM4044 |
| Assay target | TNF-alpha |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
| Assay time | 3.5-4h |
Documents
Application Note
Downloads
Navigating the Complexities of Cell-based Assays in GMP QC Testing: The Journey to Robust Assays
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