iLite® TNF-alpha Assay Ready Cells
BM3044
This iLite® TNF-α assay delivers reproducible, cell-based quantification of TNF-α bioactivity. It is ideal for functional testing of anti-TNF therapeutics and mechanism-of-action studies.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® TNF-alpha Assay Ready Cells provide a means of directly quantifying both the potency and neutralizing antibody response to TNF-alpha antagonists of diverse structure in the same assay, making it an outstanding tool for biosimilar development.
Measuring the functional activity of TNF-alpha, the assay can be used for both potency and immunogenicity assessments of TNF-alpha inhibitors in the same assay. The iLite® TNF-alpha Assay Ready Cells also provide a means of directly comparing a biosimilar and innovator product in the same assay.
The iLite TNF-alpha cells are engineered cells for use in a normalized reporter gene bioassay for screening of anti-TNF alpha activity in samples. The assay is a simple and easy test format which detects anti-TNF alpha activity and, indirectly, the presence of anti-drug antibodies.
iLite TNF-alpha cells are a genetically engineered reporter gene cell line of human origin (K562), responsive to tumor necrosis factor alpha (TNF-alpha), resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
1.5 mL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), 10% glycerol and 2.5% dimethyl sulfoxide (DMSO).
Specifications
| Product code | BM3044 |
| Assay target | TNF-alpha |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
| Assay time | 3,5-4h |
