iLite® IL-2 Assay Ready Cells
BM4002
The iLite® IL-2 assay enables sensitive quantification of IL-2 activity or inhibition. Ideal for immunotherapy development and functional assessment of IL-2-targeting biologics.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® IL-2 Assay Ready Cells is a genetically engineered reporter gene cell line optimized to respond to IL-2 with proportional expression of Firefly Luciferase. These cells allow for the testing of specific IL-2 activity and screening for unwanted inhibitory activity of IL-2.
iLite IL-2 assay ready cells are avian DT-40 cells (ATCC# CRL-2111) which have been genetically engineered and optimized to be responsive to IL-2, resulting in a proportional expression of Firefly Luciferase.
Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.
The ATCC® CRL-2111 cell line is used under a license from ATCC®
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11888).
Specifications
| Product code | BM4002 |
| Assay target | IL-2 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
