iLite® IL-12

BM4012

iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to IL-12 by specific and proportional expression of Firefly Luciferase.

The iLite IL-12 assay ready cells can be used for the quantification IL-12 or p40 inhibitor activity, or for detecting a neutralizing antibody response against such inhibitors in human serum.

The cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct under the control of a constitutive promotor.

The assay ready iLite IL-12 cells are the perfect complement to the iLite® IL-23 Assay Ready Cells, allowing testing for specific IL-12 inhibitory activity and screening for unwanted inhibitory activity of IL-23.

FOR RESEARCH USE ONLY

€ 700

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iLite® IL-12

At a glance


Product code BM4012
Assay target IL-12
Size 1 vial corresponding to one 96 well plate
Read-out of results Quantitative,
Detection system Luminescence
Regulatory status Research Use Only

What's in the box?


>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).

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