iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to IL-12 by specific and proportional expression of Firefly Luciferase.
The iLite IL-12 assay ready cells can be used for the quantification IL-12 or p40 inhibitor activity, or for detecting a neutralizing antibody response against such inhibitors in human serum.
The cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct under the control of a constitutive promotor.
The assay ready iLite IL-12 cells are the perfect complement to the iLite® IL-23 Assay Ready Cells, allowing testing for specific IL-12 inhibitory activity and screening for unwanted inhibitory activity of IL-23.
The ATCC® CRL-2111 cell line is used under a license from ATCC®
FOR RESEARCH USE ONLY
|Size||1 vial corresponding to one 96 well plate|
|Read-out of results||Quantitative,|
|Regulatory status||Research Use Only|
>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).
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