iLite® IL-12 Assay Ready Cells

BM4012

The iLite® IL-12 assay provides functional, cell-based quantification of IL-12 signaling. Suited for immune pathway studies and evaluation of therapeutic cytokine modulators.

  • High sensitivity and large dynamic range
  • "Assay-ready" format for rapid and convenient use
  • Reproducible and biologically relevant results
  • Reduced assay variability and higher reproducibility compared to cells in culture
  • Normalization of cell counts and serum matrix effects using a second reporter gene
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Product Overview

iLite® IL-12 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to IL-12 by specific and proportional expression of Firefly Luciferase.

The iLite IL-12 assay ready cells can be used for the quantification IL-12 or p40 inhibitor activity, or for detecting a neutralizing antibody response against such inhibitors in human serum.

The cells are a genetically engineered reporter gene cell line (DT-40, ATCC#CRL-2111) responsive to Interleukin-12 by specific and proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct under the control of a constitutive promotor.

The assay ready iLite IL-12 cells are the perfect complement to the iLite® IL-23 Assay Ready Cells, allowing testing for specific IL-12 inhibitory activity and screening for unwanted inhibitory activity of IL-23.

The ATCC® CRL-2111 cell line is used under a license from ATCC®

FOR RESEARCH USE ONLY

How it works

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The function of iLite assays

iLite cells have receptors on the cell surface that can bind to the marker under study.

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The function of iLite assays

Upon binding of the marker to its receptor, a signaling path activates the production of luciferase from a reporter gene.

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The function of iLite assays

Following cell lysis and the addition of a substrate, the luciferase is measured using a luminometer.

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The function of iLite assays

iLite cells contain a secondary Renilla luciferase gene that is constitutively expressed and is used for normalization.

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What's in the box?

>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).

Specifications

Product code BM4012
Assay target IL-12
Size 1 vial corresponding to one 96 well plate
Technology Cell-based reporter gene assay
Detection system Luminescence
Regulatory status Research Use Only