iLite® IL-23 Assay Ready Cells
BM4023
This iLite® IL-23 assay enables precise measurement of IL-23-induced signaling in a cell-based context. Ideal for characterizing inhibitors and monitoring potency in autoimmune drug development.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® IL-23 Assay Ready Cells are a highly specific genetically engineered reporter gene cell line based on avian DT-40 cells (ATCC#CRL2111). They are optimized to respond to IL-23 with specific, proportional expression of Firefly Luciferase without cross-reactivity to IL-12.
Lite® IL-23 Assay Ready Cells can be used as both a sensitive and specific assay for the quantification of the potency of anti-IL-23 or anti-p40 drugs. Because the cells respond specifically to IL-23, they can be used as an assay for the development of specific p19 inhibitors.
Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.
The ATCC® CRL-2111 cell line is used under a license from ATCC®
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µl of Assay Ready Cells suspended in cryoprotective medium
from Amsbio (Cat. No 11910).
Specifications
| Product code | BM4023 |
| Assay target | IL-23 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
