iLite® TLR4 Assay Ready Cells


Toll-like receptors (TLRs) are a family of pattern recognition receptors that induce pro-inflammatory responses to invading pathogens, such as bacteria, fungi, viruses, and parasites. TLR4, the most extensively studied TLR, is activated by lipopolysaccharide (LPS) – a common component of Gram-negative bacteria. In addition to LPS, a number of damage-associated molecular patterns (DAMPs), have also been shown to be capable of activating TLR4. Upon activation of TLR4, downstream signaling pathways are activated leading to the production of inflammatory cytokines and chemokines.  

iLite TLR4 Assay Ready Cells can be used to determine the activity of both TLR4 agonist and antagonist drugs, as well as any neutralizing antibodies against such drugs – all using the same assay. It is based on a human erythroleukemic cell line that has been genetically engineered and optimized for the quantification of LPS and for the detection of TLR4 inhibiting activity by specific and proportional expression of Firefly Luciferase. A second Renilla Luciferase reporter gene can be used for normalization of cell counts and to compensate for serum matrix effects.  


Add to cart    
iLite® TLR4 Assay Ready Cells
iLite® TLR4 Assay Ready Cells

At a glance

Product code BM4025
Assay target TLR4
Assay target LPS
Technology Cell-based reporter gene assay
Read-out of results Quantitative,
Detection system Luminescence
Regulatory status Research Use Only

What's in the box?

>250 µL of Assay Ready Cells suspended in cryoprotective medium


More content

Get access to scientific material, posters and more.

More downloads