iLite® IFN type I FAST Assay Ready Cells are human embryonic kidney HEK293 cells (used under a license obtained from AdVec Inc) that have been genetically engineered and optimized to be responsive to type I interferon, resulting in a proportional expression of Firefly Luciferase.
This product can be used for studies of type I interferon (IFNα and IFNβ) in test samples, including human serum.
The cells also contain a Renilla Luciferase internal standardization gene under the control of a constitutive promoter that renders assay results independent of cell number and provides a means for correcting for cytotoxic effects that may be encountered in some biological samples.
FOR RESEARCH USE ONLY
|Assay target||Type I IFN|
|Size||1 vial corresponding to one 96 well plate (1 vial (2.5mL))|
|Technology||Cell-based reporter gene assay|
|Read-out of results||Semi-quantitative,|
|Regulatory status||Research Use Only|
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).
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