iLite® GM-CSF Assay Ready Cells
BM4050
The iLite® GM-CSF assay enables functional quantification of granulocyte-macrophage colony-stimulating factor activity. Ideal for potency testing of cytokine-based biologics.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® GM-CSF Assay Ready Cells are a genetically engineered reporter gene cell line (U937) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase.
They can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum.
As a functional assay, iLite GM-CSF assay ready cells can be used to determine the activity of GM-CSF, or of GM-CSF inhibitors, as well as neutralizing antibodies, all using the same assay set-up.
The assay ready iLite GM-CSF cells Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% FBS, mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).
Specifications
| Product code | BM4050 |
| Assay target | GM-CSF |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
