The iLite® GM-CSF Assay Ready Cells can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum.
As a functional assay, iLite GM-CSF assay ready cells can be used to determine the activity of GM-CSF, or of GM-CSF inhibitors, as well as neutralizing antibodies, all using the same assay set-up.
The assay ready iLite GM-CSF cells are a genetically engineered reporter gene cell line (U937, ATCC# CRL1593.2) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
FOR RESEARCH USE ONLY
|Size||1 vial corresponding to one 96 well plate|
|Read-out of results||Quantitative,|
|Regulatory status||Research Use Only|
>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% FBS, mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).
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