iLite® IL-6 Assay Ready Cells is a genetically engineered reporter gene cell line, optimized to respond to IL-6 with proportional expression of Firefly Luciferase where the activity of IL-6 and its agonist and antagonist can be assayed in a functional and biologically relevant format.
The iLite IL-6 assay ready cells allows testing for specific IL-6 activity and screening for unwanted inhibitory activity of IL-6
The assay ready iLite IL-6 cells are based on the human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered and optimized to be responsive to human IL-6, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
FOR RESEARCH USE ONLY
|Size||1 vial corresponding to one 96 well plate|
|Read-out of results||Quantitative,|
|Regulatory status||Research Use Only|
>250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11888).
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