iLite® IL-6 Assay Ready Cells
BM4061
This reporter gene assay delivers reproducible, cell-based detection of IL-6 bioactivity — supporting the characterization of inflammatory response modulators and anti-IL-6 therapeutics.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® IL-6 Assay Ready Cells is a genetically engineered reporter gene cell line, based on the human embryonic kidney cell line, HEK293, and genetically engineered and optimized to be responsive to human IL-6. The cells' response to IL-6 results in a proportional expression of Firefly Luciferase.
These cells allow for the testing of specific IL-6 activity and screening for unwanted inhibitory activity of IL-6. The activity of IL-6 and its agonist and antagonist can be assayed in a functional and biologically relevant format.
Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11888).
Specifications
| Product code | BM4061 |
| Assay target | IL-6 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
