iLite® hCG Assay Ready Cells
BM4080
The iLite® hCG assay provides a sensitive, cell-based method for quantifying human chorionic gonadotropin bioactivity — an essential tool for potency testing of fertility and pregnancy-related products.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® hCG Assay Ready Cells can be used for the quantification of hCG activity, or neutralizing antibody response against hCG in test samples, including human serum.
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by the placenta during pregnancy and the hormone can also be used clinically to induce ovulation in the ovaries as part of a fertility treatment. hCG can be used as a tumor marker as its β subunit is secreted by certain types of cancer cells.
The assay ready iLite® hCG cells are human HEK293 cells genetically engineered and optimized to be responsive to hCG, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio (Cat. No 11888).
Specifications
| Product code | BM4080 |
| Assay target | hCG |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
