iLite® HER2 (+) Target
BM5011
HER2-expressing iLite® target cells provide a reliable platform for functional antibody testing in HER2-targeted bioassays.
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
iLite® Target HER2 (+) Assay Ready Cells are human cells based on the embryonic kidney cell line, HEK293*. They have been genetically engineered to express high and constant levels of the surface antigen HER2.
iLite® Target HER2 (+) Assay Ready Cells are optimized to give high sensitivity and specificity when used as target cells together with the iLite® ADCC and ADCP Effector Assay Ready Cells for measuring the ADCC and ADCP activity of anti-HER2 antibodies.
* The HEK-293 cell line has been used under a license obtained from AdVec Inc.
FOR RESEARCH USE ONLY
How it works
Principle of iLite® ADCC and ADCP Bioassays
Our two-cell ADCC or ADCP bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (for ADCC) or FcγRIIa (for ADCP) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM5011 |
| Assay target | ADCC ADCP HER2 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
Downloads
A Cell-Based ADCC Bioassay Comparison
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Quantification of ADCC Activity of Therapeutic Antibodies & Tolerance to Human Serum
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Designing Optimal Bioassays for Measuring ADCC & ADCP Activity
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Quantification of ADCP Activity of Therapeutic Antibodies Using Engineered Effector Cells and Target...
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A Novel Method for ADCC Activity Quantification using Engineered Effector Cells
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Navigating the Complexities of Cell-based Assays in GMP QC Testing: The Journey to Robust Assays
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The Value of Assay-Ready Cells in Providing Biologically Relevant Data for Robust Therapeutic Develo...
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How Biosimilars Are Expanding Options for Patient Care
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Biosimilar Development: Experts Discuss Analytical Characterization Toolkits for Antibody Therapeuti...
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The Future of Drug Development for Complex Pathologies: Versatile Cell-based Assays and Customizable...
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Webinar - Advancements in GMP QC Testing: A Comprehensive Guide to Cell-Based Assays
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