iLite® mTNF-alpha (+) Target
BM5013
iLite® target cells expressing membrane-bound TNF-alpha enable functional characterization of TNF-targeting biologics.
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
iLite® Target mTNFα (+) Assay Ready Cells are human cells based on the embryonic kidney cell line HEK293. They have been genetically engineered to express high and constant levels of the surface antigen mTNF-alpha.
iLite® Target mTNFα (+) Assay Ready Cells are optimized to give high sensitivity and specificity when used as target cells together with iLite® ADCC and ADCP Effector Assay Ready Cells for measuring the ADCC and ADCP activity of anti-TNF-alpha antibodies..
FOR RESEARCH USE ONLY
How it works
Principle of iLite® ADCC and ADCP Bioassays
Our two-cell ADCC or ADCP bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (for ADCC) or FcγRIIa (for ADCP) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM5013 |
| Assay target | ADCC ADCP mTNF-alpha |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
Product Flyer
