iLite® ADCC Target mTNFα (-) Assay Ready Cells are depleted of mTNFα expression, to be used as an internal control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. These control cells can also be used for adjusting background levels when analyzing serum samples, thereby effectively removing any issues with varying background between samples
iLite ADCC Target mTNF-alpha (-) Assay Ready Cells are based on a human embryonic kidney cell line, HEK293, and have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha.
The cells are for use as internal controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target mTNF-alpha (+) Assay Ready Cells when measuring the ADCC activity of anti-TNF-alpha antibodies.
FOR RESEARCH USE ONLY
Product code | BM5014 |
Assay target | ADCC |
Assay target | mTNF-alpha |
Size | 1 vial corresponding to one 96 well plate |
Read-out of results | Quantitative, |
Detection system | Luminescence |
Regulatory status | Research Use Only |
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).
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