iLite® mTNF-alpha (-) Target


iLite® ADCC Target mTNFα (-) Assay Ready Cells are depleted of mTNFα expression, to be used as an internal control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. These control cells can also be used for adjusting background levels when analyzing serum samples, thereby effectively removing any issues with varying background between samples

iLite ADCC Target mTNF-alpha (-) Assay Ready Cells are based on a human embryonic kidney cell line, HEK293, and have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha.

The cells are for use as internal controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target mTNF-alpha (+) Assay Ready Cells when measuring the ADCC activity of anti-TNF-alpha antibodies.


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iLite® mTNF-alpha (-) Target
iLite® mTNF-alpha (-) Target

At a glance

Product code BM5014
Assay target ADCC
Assay target mTNF-alpha
Size 1 vial corresponding to one 96 well plate
Read-out of results Quantitative,
Detection system Luminescence
Regulatory status Research Use Only

What's in the box?

>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).


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