iLite® mTNF-alpha (-) Target
BM5014
A control cell line lacking membrane-bound TNF-alpha, used for specificity assessment in TNF-targeted assays.
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
iLite® Target mTNF-alpha (-) Assay Ready Cells are human cells based on the embryonic kidney cell line, HEK293. They have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha.
These cells are for use as internal controls for unspecific activation of ADCC and ADCP, which can be a confounding factor when performing ADCC and ADCP assays. They can also be used for adjusting background levels when analyzing serum samples, effectively removing any issues with varying background between samples
iLite® Target mTNF-alpha (-) Assay Ready Cells are optimized to be used together with iLite® ADCC and ADCP Effector Assay Ready Cells and iLite® Target mTNF-alpha (+) Assay Ready Cells for measuring the ADCC and ADCP activity of anti-TNF-alpha antibodies.
FOR RESEARCH USE ONLY
How it works
Principle of iLite® ADCC and ADCP Bioassays
Our two-cell ADCC or ADCP bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (for ADCC) or FcγRIIa (for ADCP) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).
Specifications
| Product code | BM5014 |
| Assay target | ADCC ADCP mTNF-alpha |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
Product Flyer
