iLite® HER2 (-) Target
BM5016
This HER2-negative iLite® target cell line serves as a critical control in HER2 bioactivity and ADCC assays.
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
iLite Target HER2 (-) Assay Ready Cells are based on the human embryonic kidney cell line, HEK293*, and have been genetically engineered and optimized to be depleted of HER2 expression.
These cells are to be used as an internal control. The control cells can also be used for adjusting background levels when analyzing serum samples, effectively removing any issues with varying background between samples.
The cells are optimized to be use together with iLite ADCC and ADCP Effector Assay Ready Cells and iLite Target HER2 (+) Assay Ready Cells for measuring the ADCC and ADCP activity of anti-HER2 antibodies.
* The HEK-293 cell line has been used under a license obtained from AdVec Inc.
FOR RESEARCH USE ONLY
How it works
Principle of iLite® ADCC and ADCP Bioassays
Our two-cell ADCC or ADCP bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (for ADCC) or FcγRIIa (for ADCP) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.
What's in the box?
>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).
Specifications
| Product code | BM5016 |
| Assay target | ADCC ADCP HER2 |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
Documents
Application Note
