iLite® mVEGF (-) Target
BM5018
Negative control cell line for VEGF-targeted assays, ensuring reliable discrimination of specific versus non-specific activity.
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
Product Overview
iLite® ADCC Target mVEGF (-) Assay Ready Cells can be used for assessing the possibility of an anti-VEGF antibody drug inducing ADCC.
iLite® mVEGF (-) Target Assay Ready Cells are based on the human embryonic kidney cell line HEK2931*, and have been genetically engineered to not express any membrane bound VEGF.
The cells are for use as internal controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® mVEGF (+) Target Assay Ready Cells for measuring the ADCC activity of anti-VEGF antibodies.
* The HEK-293 cell line has been used under a license obtained from AdVec Inc.
FOR RESEARCH USE ONLY
How it works
Principle of iLite® ADCC Bioassays
Our two-cell ADCC bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (CD16) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.
What's in the box?
>250 μL of Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010)
Specifications
| Product code | BM5018 |
| Assay target | mVEGF ADCC |
| Size | 1 vial corresponding to one 96 well plate |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
