iLite® CD19 (-) Target Assay Ready Cells

BM5026

This CD19-negative iLite® target cell line serves as a specificity control in ADCC and T cell engager assays.

  • "Assay-ready" format for rapid and convenient use
  • Reproducible and biologically relevant results
  • Reduced assay variability and higher reproducibility compared to cells in culture
Add to quote
iLite® CD19 (-) Target Assay Ready Cells

Product Overview

iLite® CD19 (-) Target Assay Ready Cells are homologous to the target CD19 (+) cell line and can be used as a negative control in assay assessing antibodies directed towards CD19.

Lite® CD19 (-) Target Assay Ready Cells are genetically engineered human cells (Raji, ATCC# CCL-86) depleted of CD19 expression.

The cells can be used as a target negative control together with iLite® CD3 Effector Assay Ready Cells for the measurement of bi-specific CD3-CD19 antibody activity.

FOR RESEARCH USE ONLY

How it works

arrow
Principle of iLite® ADCC Bioassays

Principle of iLite® ADCC Bioassays

Our two-cell ADCC bioassays consist of target cells expressing the antigen under study and effector cells closely resembling the natural FcγRIIIa (CD16) signal transduction pathway. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.

Principle of iLite® ADCC Bioassays

Principle of iLite® ADCC Bioassays

A therapeutic antibody can bind to its antigen on the target cell surface. The fc-region of the antibody can bind to the FcγRIIIa receptor on the effector cells.

Principle of iLite® ADCC Bioassays

Principle of iLite® ADCC Bioassays

The antibody binding to the target and effector cells triggers a signal pathway in the effector cell, activating the Firefly luciferase gene. The strength of the resulting luciferase luminescence correlates with the antibody’s ability to activate ADCC.

arrow

How it works

arrow
Principle of iLite® T-cell activation bioassays

Principle of iLite® T-cell activation bioassays

Our two-cell T-cell activation bioassays consist of target cells expressing the antigen under study and effector cells expressing CD3/TCR Complex. Target control cells that do not express the antigen are also available. The effector cells contain two luciferase genes: an inducible Firefly luciferase gene and a constitutively expressed Renilla luciferase gene used for control purposes.

Principle of iLite® T-cell activation bioassays

Principle of iLite® T-cell activation bioassays

The T-cell activation process is triggered when the effector cell interacts with a bispecific drug antibody (BsAb) bound to its target cell. The CD3 complex on the surface of the effector cell binds to the CD3 part of the antibody, thus creating a bridge between the effector and target cells.Following the formation of this bridge, the engineered effector cells will, instead of activating the T-cell as in the in vivo situation, produce luciferase through an intracellular pathway and generate luminescence exclusively from this cross-linking and signaling. The strength of the luminescence correlates with the ability of the drug to activate the T-cells.

arrow

What's in the box?

>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium.

Specifications

Product code BM5026
Assay target CD19 ADCC
Technology Cell-based reporter gene assay
Detection system Luminescence
Regulatory status Research Use Only

Documents