iLite® TLR9 Assay Ready Cells
BM6069
This iLite® TLR9 reporter assay provides cell-based measurement of TLR9 pathway activation. Supports the discovery and characterization of CpG oligonucleotides and TLR9 modulators.
- High sensitivity and large dynamic range
- "Assay-ready" format for rapid and convenient use
- Reproducible and biologically relevant results
- Reduced assay variability and higher reproducibility compared to cells in culture
- Normalization of cell counts and serum matrix effects using a second reporter gene
Product Overview
iLite® TLR9 Assay Ready Cells are based on the HEK293 cells that have been genetically engineered and optimized to be responsive to unmethylated CpG islands through TLR9, resulting in a proportional expression of Firefly Luciferase. They represent a functional, robust, and agile technology to facilitate the path towards the development of new compounds to activate or inhibit TLR9.
Normalization of cell count and serum matrix effects are obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promoter.
iLite TLR9 Assay Ready Cells can be used to quantify class B CpG oligonucleotides and evaluate the inhibiting activity of new compounds.
Toll-like receptor 9 (TLR9) is an innate immune sensor for DNA, as it recognizes unmethylated CpG motifs. TLR9 agonists have substantial potential as vaccine adjuvants, and their activation has also been related to immunotoxicity against AAV vectors.
FOR RESEARCH USE ONLY
How it works
The function of iLite assays
iLite cells have receptors on the cell surface that can bind to the marker under study.
What's in the box?
>250 µL of Assay Ready Cells suspended in cryoprotective medium.
Specifications
| Product code | BM6069 |
| Assay target | TLR9 Class B CpG Oligonucleotides |
| Technology | Cell-based reporter gene assay |
| Detection system | Luminescence |
| Regulatory status | Research Use Only |
| Assay time | 6h |
Documents
Application Note
Downloads
Navigating the Complexities of Cell-based Assays in GMP QC Testing: The Journey to Robust Assays
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Webinar - Advancements in GMP QC Testing: A Comprehensive Guide to Cell-Based Assays
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