Complement C4d


The Complement C4d assay is a highly specific C4d ELISA kit for measurement of C4d in human plasma. The assay targets a unique cleavage epitope on C4d, which means that only the C4d is measured.

The C4d protein is a product of the classical and the lectin pathways and is increasingly recognized as a potential biomarker for diseases where antibodies can cause tissue damage, such as systemic autoimmune diseases.

C4d has the potential to detect patients at risk for the consequences of antibody-mediated disease and is also used during the development of new therapeutics that block complement activation to identify and monitor patients who may possibly benefit from these drugs.

For custom requests, please contact an expert.


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Complement C4d - RUO
Complement C4d - RUO

At a glance

Product code COMPL C4d RUO
Assay target C4d
Size 96 wells
Technology ELISA
Read-out of results Semi-quantitative,
Range 0-400 ng/ml
Assay time 2h
Detection system HRP/TMB read at 450 nm
Regulatory status Research Use Only

What's in the box?

  • One frame with micro titer wells (12x8)
  • 1,5mL Low control
  • 1,5mL High control
  • 2x30 mL Diluent
  • 15 mL Conjugate
  • 15 mL Substrate TMB
  • 15 mL Stop solution
  • 30 mL Wash solution, 30x concentrated
  • 6 x 1,5mL vials with calibrators containing human recombinant C4d - 0 ng/mL, 5ng/mL, 25 ng/mL, 100ng/mL, 200ng/mL and 400ng/mL

Please note: All reagents in the kit are ready to use except the wash solution. The reagents should be stored at 2-8°C. Components from different lots shall not be mixed. When stored at 2-8°C the diluted wash solution is stable until the date of expiration of the kit.


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