Complement Factor P Functional Assay


The Factor P Functional assay is an enzyme-linked immunosorbent assay (ELISA) that combines principles of the functional complement activity assays with the use of labelled antibodies specific for deposited complement proteins. The amount of deposited complement proteins is proportional to the functional activity of Factor P in the sample.

The purpose of the product is to determine the function of Factor P in test samples. The functionality is determined qualitatively in relation to the calibrator (human serum) set to be 100% functional.

Want to know more about the principle of the functional Factor P assay? Click to view an animation.

FOR RESEARCH USE ONLY. Not for use as a diagnostic tool or in the management of patients.

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Complement Factor P Functional Assay

At a glance

Product code COMPL FPF RUO
Assay target Properdin
Size 96 well
Technology ELISA
Read-out of results Qualitative, Semi-quantitative,
Range 0 - 200% relative activity
Assay time 4h
Detection system HRP/TMB read at 450 nm
Regulatory status Research Use Only

What's in the box?

  • One frame with microtiter wells (12x8) 
  • 1.5 mL Negative control (NC)
  • 3.0 mL Calibrator, 200 ng/mL
  • 32 mL Diluent (Dil), red color
  • 30 mL Wash solution, 30x concentrated
  • Activator, lyophilized
  • 15 mL Activator Diluent
  • 3.0 mL Quench
  • 7.5 mL Conjugate containing HRP-labelled antibody directed against C3b
  • 15 mL Substrate TMB
  • 15 mL Stop solution (0.5M H2SO4)

Please note: All reagents in the kit are ready for use except washing solution and activator. All reagents should be stored at 2-8°C, except for the Activator. When stored at 2-8°C, the diluted wash solution is stable until the date of expiration for the kit.


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