INDIVIDUAL TEST 023

dsDNA Antibodies (IgG)

Indication

Suspicion of systemic lupus erythematosus(SLE) or incipient exacerbation of this disease, chronic aggressive hepatitis of the “lupoid hepatitis” (autoimmune hepatitis) type.

Sample material

Serum

• Minim. volume: 0,5 mL

Transport

Within Sweden

• room temperature

International

• cold

Method

Indirect immunofluorescence (IIF) on Crithidia luciliae

Reference interval

<1:10 negative

Result

Results are reported as negative or positive with a fluorescence intensity. Positive results will be titrated.

Interpretation

IgG antibodies against dsDNA are a strong marker for systemic lupus erythematosus (SLE) and are associated with SLE-nephritis but can also be seen in autoimmune hepatitis type 1. In SLE, a rise in antibody level often indicates increased disease activity, while levels often drop under remission.

References

• Enocsson H et al. J Rheumatol. 2015. Four Anti-dsDNA Antibody Assays in Relation to Systemic Lupus Erythematosus Disease Specificity and Activity. PMID: 25684763
• Wiik A et al. Lupus. 2006. European attempts to set guidelines for improving diagnostics of autoimmune rheumatic disorders.;15(7):391-6. doi: 10.1191/0961203306lu2322oa. PMID: 16898171
• Yee CS et al. Arthritis Rheum. 2007. British Isles Lupus Assessment Group 2004 index is valid for assessment of disease activity in systemic lupus erythematosus. PMID: 18050213
• Hamann D, Smeenk RJT. dsDNA autoantibodies. In: Shoenfeld Y, Gershwin ME, Meroni PL, editors. Autoantibodies. London: Elsevier; 2006. p. 159-67.
• Ho A et al. Arthritis Rheum. 2001. Decreases in anti-double-stranded DNA levels are associated with concurrent flares in patients with systemic lupus erythematosus. PMID: 11665975
• Swaak T, Smeenk R. Ann Rheum Dis. 1985. Detection of anti-dsDNA as a diagnostic tool: a prospective study in 441 non-systemic lupus erythematosus patients with anti-dsDNA antibody (anti-dsDNA). PMID: 3872637
• Anti-Nuclear Factor Serum (Homogeneous) Human NIBSC code: 66/233 Instructions for use (Version 6.0, Dated 11/04/2008)

Last updated: 2024-06-26