Performance Data

iLite® VEGF Performance Data

The iLite® VEGF Assay Ready Cells have been genetically engineered to specifically detect VEGF activity and can be used to measure the potency of VEGF inhibitor drugs, as well as neutralizing antibodies against such drugs in human serum. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

In this performance data file, we present data for the VEGF cell line generated throughout the quality assessment process of the product. This data shows that the assays are reliable, accurate, and reproducible - and helps keep errors resulting from biological variation and methodology at a minimum.

Performance Data

iLite® VEGF Performance Data

The iLite® VEGF Assay Ready Cells have been genetically engineered to specifically detect VEGF activity and can be used to measure the potency of VEGF inhibitor drugs, as well as neutralizing antibodies against such drugs in human serum. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

In this performance data file, we present data for the VEGF cell line generated throughout the quality assessment process of the product. This data shows that the assays are reliable, accurate, and reproducible - and helps keep errors resulting from biological variation and methodology at a minimum.

LEARN MORE ABOUT

The most common bioanalytical aspects of the iLite VEGF cell-based reporter gene assay

The cell-based iLite®  VEGF assay is a cleverly designed, sensitive, and specific reporter-gene assay with firefly luciferase readout intended to be used in various applications throughout the drug development continuum.

The assay allows an easy, rapid, and accurate test format for a wide range of applications – including the determination of neutralizing antibodies against VEGF and the quantification of VEGF.

Included chapters:

  • Cell line Characterization
    - Validation of manufacturing process & determination of the overall cell line variation
    - Real-time stability study
    - Dynamic range assay application example
  • Bioassay variation & performance
    - Accuracy & variations – day-to-day
    - Accuracy & variations – lot-to-lot
    - Linearity (parallelism) functional / linearity (parallelism) inhibitory
    - Plate homogeneity
  • Cell line bioassay pre-clinical & clinical applications
    - Immunogenicity characterization


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