WIESLAB® Complement System Screen - RUO

COMPL300RUO

A RUO-format screening assay that evaluates activation of the classical, lectin, and alternative pathways — supporting discovery research and mechanistic studies in complement biology.

  • Reliable, objective, and easy-to-interpret results
  • Results in just a few hours
  • Low inter-assay variability and excellent reproducibility
  • Developed by our in-house experts in collaboration with key opinion leaders
  • Ready-to-use reagents for a streamlined workflow
  • Excellent specificity means clear results without false positives
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WIESLAB® Complement System Screen - RUO

Product Overview

The Wieslab® Complement system Screen kit is an enzyme immunoassay for the qualitative determination of functional classical, MBL, and alternative complement pathways determination and determination of complement deficiencies in human serum. Non-human primate and swine samples can also be assayed with the Wieslab® Complement system Screen.

The assay combines the specific activation of each pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. The amount of C5b-9 generated is proportional to the functional activity of complement pathways.

The Wieslab Complement assays have been published as a useful tool in the evaluation of complement therapeutics, in the clinic, clinical trials, and in drug development.

FOR RESEARCH USE ONLY

This product is also available for in vitro diagnostic use - Product code: COMPL300

What's in the box?

  • One frame with break apart wells (4x8x3) - 4 strips for classical pathway (CP), 4 strips for MBL pathway (MP), 4 strips for alternative pathway (AP)
  • 10 ml Diluent CP
  • 10 ml Diluent MP
  • 10 ml Diluent AP
  • 13 ml Conjugate
  • 13 ml Substrate solution
  • 30 ml Wash solution 30x concentrated
  • 0,2 ml Negative control (NC) containing human serum
  • 0,2 ml Positive control (PC) containing freezed dried human serum

Plase note! All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control should be stored at -20° C.

Specifications

Product code COMPL300RUO
Size 96 wells
Technology ELISA
Detection system ALP/pNPP read at 405 nm
Regulatory status Research Use Only
Assay time 2h