WIESLAB® Complement System Alternative Pathway
COMPLAP330
Analyze specific activation of the alternative pathway with this functional ELISA, ideal for investigating complement involvement in immune disorders and therapeutic development.
- Reliable, objective, and easy-to-interpret results
- Results in just a few hours
- Low inter-assay variability and excellent reproducibility
- Developed by our in-house experts in collaboration with key opinion leaders
- Ready-to-use reagents for a streamlined workflow
- Excellent specificity means clear results without false positives
Product Overview
The Wieslab® Complement System Alternative Pathway kit is an immunoassay for the qualitative determination of functional alternative complement pathway, and the determination of complement deficiencies in human serum.
The Wieslab Complement assay combines the specific activation of the pathway with the use of labeled antibodies specific for C5b-9, a neoepitope of the terminal complement complex produced as the result of complement activation. The amount of C5b-9 generated is proportional to the functional activity of the complement system. The convenient ELISA technology has several advantages compared to the traditional hemolytic assays.
This product is also available as research use only - Product code: COMPL AP330 RUO.
What's in the box?
- One frame with break-apart wells (12x8)
- 35 mL Diluent AP
- 13 mL Conjugate
- 13 mL Substrate solution
- 30 mL Wash solution 30x concentrated
- 0,2 mL Negative control (NC) containing human serum
- 0,2 mL Positive control (PC) containing freezed dried human serum
Please note: All reagents in the kit are ready for use except washing solution and controls. The reagents should be stored at 2-8° C except the positive control. The positive control must be stored at -20°C.
Specifications
| Product code | COMPLAP330 |
| Size | 96 wells |
| Technology | ELISA |
| Detection system | ALP/pNPP read at 405 nm |
| Regulatory status | IVD, CE labeled |
| Assay time | 2h |