WIESLAB® Complement System Classical Pathway

COMPLCP310

Quantify the activation of the classical complement pathway with this functional ELISA—an essential tool for immune monitoring, biomarker discovery, and complement-targeted research.

  • Reliable, objective, and easy-to-interpret results
  • Results in just a few hours
  • Low inter-assay variability and excellent reproducibility
  • Developed by our in-house experts in collaboration with key opinion leaders
  • Ready-to-use reagents for a streamlined workflow
  • Excellent specificity means clear results without false positives
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WIESLAB® Complement System Classical Pathway

Product Overview

The Wieslab® Complement System Classical Pathway is an immunoassay for the qualitative and/or semi-quantitative determination of functional classical complement pathway and for the determination of complement deficiencies in human serum.

The Wieslab Complement Classical Pathway assay combines the specific activation of the pathway with the use of labeled antibodies specific for C5b-9, a neoepitope of the terminal complement complex produced as the result of complement activation.

The objective quantification of the activity of the classical pathway is valuable in a number of different clinical situations. The amount of complement activation can be expressed either in a qualitative way relative the value of a positive control or in a semi-quantitative way using a calibrator curve derived from a separate lyophilized activity control.

This product is also available as research use only - Product code: COMPL CP310 RUO.

What's in the box?

  • One frame with break-apart wells (12x8)
  • 2 x 35 mL Diluent CP
  • 13 mL Conjugate
  • 13 mL Substrate solution
  • 30 mL Wash solution 30x concentrated
  • 0,2 mL Negative control (NC) containing human serum
  • Lyophilized positive control (PC) containing human serum
  • Lyophilized activity control (AC) for semi-quantitative application, containing human serum

Please note: All reagents in the kit are ready to use except wash solution and controls. The reagents should be stored at 2-8° C. The positive control and the activity control should be stored at -20° C upon arrival. The reconstituted positive control and activity control should be stored at –70° C and may be thawed once.

Specifications

Product code COMPLCP310
Size 96 wells
Technology ELISA
Detection system ALP/pNPP read at 405 nm
Regulatory status IVD, CE labeled
Assay time 2h