What is the assay time for the WIESLAB® Complement System Classical Pathway - RUO?

The estimated assay time is 2h.

What technology and detection system does the WIESLAB® Complement System Classical Pathway - RUO use?

This assay utilizes ELISA technology, with a ALP/pNPP read at 405 nm detection system.

What is the format or size of the WIESLAB® Complement System Classical Pathway - RUO?

This kit is supplied in a 96 wells format.

What is the regulatory status of WIESLAB® Complement System Classical Pathway - RUO?

The regulatory status is Research Use Only.

What is included in the WIESLAB® Complement System Classical Pathway - RUO kit?

The kit includes: One frame with break-apart wells (12x8) ; 2 x 35 mL Diluent CP; 13 mL Conjugate ; 13 mL Substrate solution ; 30 mL Wash solution 30x concentrated ; 0,2 mL Negative control (NC) containing human serum; Lyophilized positive control (PC) containing human serum; Lyophilized activity control (AC) for semi-quantitative application, containing human serum; Please note: All reagents in the kit are ready to use except wash solution and controls. The reagents should be stored at 2-8° C. The positive control and the activity control should be stored at -20° C upon arrival. The reconstituted positive control and activity control should be stored at –70° C and may be thawed once.

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WIESLAB® Complement System Classical Pathway - RUO

COMPLCP310RUO

RUO ELISA enabling sensitive detection of classical pathway activation to support exploratory research into autoimmune and inflammatory disease models.

Reliable, objective, and easy-to-interpret results
Results in just a few hours
Low inter-assay variability and excellent reproducibility
Developed by our in-house experts in collaboration with key opinion leaders
Ready-to-use reagents for a streamlined workflow
Excellent specificity means clear results without false positives

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WIESLAB® Complement System Classical Pathway - RUO

Product Overview

The Wieslab® Complement System Classical Pathway is an enzyme immunoassay for the qualitative and/or semi-quantitative determination of functional classical complement pathway and determination of complement deficiencies in human serum. Non-human primate and swine samples can also be assayed with Wieslab® Complement System Classical Pathway.

The Wieslab Complement System Classical Pathway assay combines the specific activation of the pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. The amount of complement activation can be expressed either in a qualitative way relative the value of a positive control or in a semi-quantitative way using a calibrator curve derived from a separate lyophilized activity control.

The Wieslab Complement assays have been reported in literature as useful tools in the evaluation of complement therapeutics, in clinic, clinical trials and in drug development.

FOR RESEARCH USE ONLY

This product is also available for in vitro diagnostic use - Product code: COMPL CP310

What's in the box?

One frame with break-apart wells (12x8)
2 x 35 mL Diluent CP
13 mL Conjugate
13 mL Substrate solution
30 mL Wash solution 30x concentrated
0,2 mL Negative control (NC) containing human serum
Lyophilized positive control (PC) containing human serum
Lyophilized activity control (AC) for semi-quantitative application, containing human serum

Please note: All reagents in the kit are ready to use except wash solution and controls. The reagents should be stored at 2-8° C. The positive control and the activity control should be stored at -20° C upon arrival. The reconstituted positive control and activity control should be stored at –70° C and may be thawed once.