WIESLAB® Complement Classical Pathway (RUO)
COMPLCP310RUO
The Wieslab® Complement System Classical Pathway is an enzyme immunoassay for the qualitative and/or semi-quantitative determination of functional classical complement pathway and determination of complement deficiencies in human serum. Non-human primate and swine samples can also be assayed with Wieslab® Complement System Classical Pathway.
The Wieslab Complement System Classical Pathway assay combines the specific activation of the pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. The amount of complement activation can be expressed either in a qualitative way relative the value of a positive control or in a semi-quantitative way using a calibrator curve derived from a separate lyophilized activity control.
The Wieslab Complement assays have been published as useful tools in evaluation of complement therapeutics, in clinic, clinical trials and in drug development.
FOR RESEARCH USE ONLY
This product is also available for in vitro diagnostic use - Product code: COMPL CP310
At a glance
| Product code | COMPLCP310RUO |
| Size | 96 wells |
| Technology | ELISA |
| Read-out of results | Qualitative, Semi-quantitative, |
| Assay time | 2h |
| Detection system | ALP/pNPP read at 405 nm |
| Regulatory status | Research Use Only |
What's in the box?
- One frame with break-apart wells (12x8)
- 2 x 35 mL Diluent CP
- 13 mL Conjugate
- 13 mL Substrate solution
- 30 mL Wash solution 30x concentrated
- 0,2 mL Negative control (NC) containing human serum
- Lyophilized positive control (PC) containing human serum
- Lyophilized activity control (AC) for semi-quantitative application, containing human serum
Please note: All reagents in the kit are ready to use except wash solution and controls. The reagents should be stored at 2-8° C. The positive control and the activity control should be stored at -20° C upon arrival. The reconstituted positive control and activity control should be stored at –70° C and may be thawed once.
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